Anti-UGGT/UGT1 antibody (ab241357)
Key features and details
- Rabbit polyclonal to UGGT/UGT1
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-UGGT/UGT1 antibody
See all UGGT/UGT1 primary antibodies -
Description
Rabbit polyclonal to UGGT/UGT1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human UGGT/UGT1 aa 1505-1555. The exact sequence is proprietary.
Database link: Q9NYU2 -
Positive control
- WB:HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to UGGT/UGT1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab241357 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at 2-10 µg/mg of lysate.
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WB |
1/2000 - 1/10000. Predicted molecular weight: 177 kDa.
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Notes |
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IP
Use at 2-10 µg/mg of lysate. |
WB
1/2000 - 1/10000. Predicted molecular weight: 177 kDa. |
Target
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Function
Recognizes glycoproteins with minor folding defects. Reglucosylates single N-glycans near the misfolded part of the protein, thus providing quality control for protein folding in the endoplasmic reticulum. Reglucosylated proteins are recognized by calreticulin for recycling to the endoplasmic reticulum and refolding or degradation. -
Tissue specificity
Higher levels in pancreas, skeletal muscle, kidney, and brain. Low levels in lung and heart. -
Pathway
Protein modification; protein glycosylation. -
Sequence similarities
Belongs to the glycosyltransferase 8 family. -
Domain
The N-terminal non-catalytic domain is assumed to mediate recognition of proteins with partial folding defects. -
Cellular localization
Endoplasmic reticulum lumen. Endoplasmic reticulum-Golgi intermediate compartment. - Information by UniProt
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Database links
- Entrez Gene: 56886 Human
- Entrez Gene: 320011 Mouse
- Omim: 605897 Human
- SwissProt: Q9NYU2 Human
- SwissProt: Q6P5E4 Mouse
- Unigene: 34180 Human
- Unigene: 261022 Mouse
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Alternative names
- GT antibody
- HUGT1 antibody
- UDP glucose glycoprotein glucosyltransferase 1 antibody
see all
Images
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All lanes : Anti-UGGT/UGT1 antibody (ab241357) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Predicted band size: 177 kDa
Exposure time: 10 secondsLysates prepared in NETN buffer.
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UGGT/UGT1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241357 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241357 at 1 µg/ml.
Lane 1: ab241357 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.Detection: Chemiluminescence with exposure time of 30 seconds.
Lysates prepared in NETN buffer.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab241357 has not yet been referenced specifically in any publications.