Overview

  • Product name

    Anti-UGGT/UGT1 antibody [EPR5105]
    See all UGGT/UGT1 primary antibodies
  • Description

    Rabbit monoclonal [EPR5105] to UGGT/UGT1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human UGGT/UGT1 aa 1500-1600.
    Database link: Q9NYU2

  • Positive control

    • Human skeletal muscle, BXPC-3, SH-SY5Y, and MCF7 lysates, Human brain tissue
  • General notes

     

     

     This product was previously labelled as UGGT

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR5105
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 170 kDa (predicted molecular weight: 177 kDa).
IHC-P 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      Recognizes glycoproteins with minor folding defects. Reglucosylates single N-glycans near the misfolded part of the protein, thus providing quality control for protein folding in the endoplasmic reticulum. Reglucosylated proteins are recognized by calreticulin for recycling to the endoplasmic reticulum and refolding or degradation.
    • Tissue specificity

      Higher levels in pancreas, skeletal muscle, kidney, and brain. Low levels in lung and heart.
    • Pathway

      Protein modification; protein glycosylation.
    • Sequence similarities

      Belongs to the glycosyltransferase 8 family.
    • Domain

      The N-terminal non-catalytic domain is assumed to mediate recognition of proteins with partial folding defects.
    • Cellular localization

      Endoplasmic reticulum lumen. Endoplasmic reticulum-Golgi intermediate compartment.
    • Information by UniProt
    • Database links

    • Alternative names

      • GT antibody
      • HUGT1 antibody
      • UDP glucose glycoprotein glucosyltransferase 1 antibody
      • UDP Glucose Glycoprotein Glucosyltransferase antibody
      • UDP--Glc:glycoprotein glucosyltransferase antibody
      • UDP-glucose ceramide glucosyltransferase-like 1 antibody
      • UDP-glucose:glycoprotein glucosyltransferase 1 antibody
      • UGCGL1 antibody
      • UGGG1 antibody
      • UGGG1_HUMAN antibody
      • UGGT antibody
      • Uggt1 antibody
      • UGT1 antibody
      • UGTR antibody
      see all

    Images

    • Staining of UGGT/UGT1 in Paraffin-embedded Human brain tissue by Immunohistochemistry using ab124879 at a dilution of 1/100.

    • All lanes : Anti-UGGT/UGT1 antibody [EPR5105] (ab124879) at 1/10000 dilution

      Lane 1 : Human skeletal muscle lysate
      Lane 2 : BXPC-3 cell lysate
      Lane 3 : SH-SY5Y cell lysate
      Lane 4 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 177 kDa

    References

    This product has been referenced in:

    • Ilca FT  et al. TAPBPR mediates peptide dissociation from MHC class I using a leucine lever. Elife 7:N/A (2018). Read more (PubMed: 30484775) »
    • Neerincx A  et al. TAPBPR bridges UDP-glucose:glycoprotein glucosyltransferase 1 onto MHC class I to provide quality control in the antigen presentation pathway. Elife 6:N/A (2017). Read more (PubMed: 28425917) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab124879.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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