Overview

  • Product name
    Anti-UGPase antibody [EPR10627(B)]
    See all UGPase primary antibodies
  • Description
    Rabbit monoclonal [EPR10627(B)] to UGPase
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
  • Immunogen

    Synthetic peptide corresponding to Human UGPase (internal sequence).
    Database link: Q16851

  • Positive control
    • HepG2; Hela; NIH 3T3 cell lysates; Human fetal liver tissue lysate; Human colon adenocarcinoma; kidney and NIH 3T3 cells.
  • General notes

     

     

    Previously labelled as UGP2. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab154817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 57 kDa.
IHC-P 1/50 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function
      Plays a central role as a glucosyl donor in cellular metabolic pathways.
    • Sequence similarities
      Belongs to the UDPGP type 1 family.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • pHC379 antibody
      • UDP glucose diphosphorylase antibody
      • UDP glucose pyrophosphorylase 1 antibody
      • UDP glucose pyrophosphorylase 2 antibody
      • UDP glucose pyrophosphorylase antibody
      • UDP-glucose pyrophosphorylase antibody
      • UDPG antibody
      • UDPGP 2 antibody
      • UDPGP antibody
      • UDPGP2 antibody
      • UGP 1 antibody
      • UGP 2 antibody
      • UGP1 antibody
      • Ugp2 antibody
      • UGPA_HUMAN antibody
      • UGPase 2 antibody
      • UGPase antibody
      • UGPP 1 antibody
      • UGPP 2 antibody
      • UGPP1 antibody
      • UGPP2 antibody
      • Uridyl diphosphate glucose pyrophosphorylase 1 antibody
      • Uridyl diphosphate glucose pyrophosphorylase 2 antibody
      • UTP glucose 1 phosphate uridyltransferase antibody
      • UTP glucose 1 phosphate uridylyltransferase 2 antibody
      • UTP glucose 1 phosphate uridylyltransferase antibody
      • UTP--glucose-1-phosphate uridylyltransferase antibody
      see all

    Images

    • All lanes : Anti-UGPase antibody [EPR10627(B)] (ab154817) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : NIH 3T3 cell lysate
      Lane 4 : Human fetal liver tissue lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 57 kDa

    • Immunohistochemical analysis of paraffin-embedded Human colon adenocarcinoma labeling UGPase using ab154817 at 1/50 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human kidney labeling UGPase using ab154817 at 1/50 dilution.

    • Flow cytometric analysis of permeabilized NIH 3T3 cells labeling UGPase using ab154817 (red) at 1/10 dilution or a rabbit IgG (negative) (green).

    • Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue using ab154817 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human normal liver tissue using ab154817 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human normal heart tissue using ab154817 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human normal colon tissue using ab154817 showing +ve staining.

    • Lanes 1-2 : Anti-UGPase antibody [EPR10627(B)] (ab154817) at 1/10000 dilution
      Lanes 3-4 : Anti-UGPase antibody [EPR10627(B)] (ab154817) at 1/20000 dilution


      All lanes : Pig muscle sarcoplasm tissue lysate

      Lysates/proteins at 40 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit IgG (H+L) polyclonal at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 57 kDa
      Observed band size: 45-47 kDa
      why is the actual band size different from the predicted?


      Exposure time: 5 minutes

      See Abreview

    References

    This product has been referenced in:
    • Zhang Y  et al. GLUT1 and lactose synthetase are critical genes for lactose synthesis in lactating sows. Nutr Metab (Lond) 15:40 (2018). Read more (PubMed: 29946342) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Pig Tissue lysate - other (Muscle Sarcoplasm)
    Gel Running Conditions
    Reduced Denaturing (12.5% Acrylamide)
    Loading amount
    40 µg
    Specification
    Muscle Sarcoplasm
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Dr. Shannon Cruzen

    Verified customer

    Submitted Sep 30 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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