Overview

  • Product name

  • Description

    Rabbit polyclonal to UGT1A1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Rabbit, Horse, Cow, Cat, Dog, Pig
  • Immunogen

    A 14-22 amino acid sequence within synthetic peptide: VWLFRSDFVK DYPRPIMPNM VFVGGINCLH QNPLSQEFEA YINASGEHGI, corresponding to internal sequence amino acids 253-302 of Human UGT1A1

  • Positive control

    • Fetal liver cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab62600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Information by UniProt
  • Database links

  • Alternative names

    • BILIQTL1 antibody
    • Bilirubin specific UDPGT isozyme 1 antibody
    • bilirubin UDP glucuronosyltransferase 1 1 antibody
    • bilirubin UDP glucuronosyltransferase isozyme 1 antibody
    • Bilirubin-specific UDPGT isozyme 1 antibody
    • EC 2.4.1.17 antibody
    • GNT1 antibody
    • HUG BR1 antibody
    • HUG-BR1 antibody
    • HUGBR1 antibody
    • PHENOL/BILIRUBIN UDP GLUCURONOSYLTRANSFERASE antibody
    • UD11_HUMAN antibody
    • UDP glucuronosyltransferase 1 1 [Precursor] antibody
    • UDP glucuronosyltransferase 1 family polypeptide A1 antibody
    • UDP glucuronosyltransferase 1A1 antibody
    • UDP GLYCOSYLTRANSFERASE 1 antibody
    • UDP-glucuronosyltransferase 1-1 antibody
    • UDP-glucuronosyltransferase 1-A antibody
    • UDP-glucuronosyltransferase 1A1 antibody
    • UDPGT antibody
    • UDPGT 1-1 antibody
    • UGT 1A antibody
    • UGT-1A antibody
    • UGT1 antibody
    • UGT1 01 antibody
    • UGT1*1 antibody
    • UGT1-01 antibody
    • UGT1.1 antibody
    • UGT1A antibody
    • Ugt1a1 antibody
    • URIDINE DIPHOSPHATE GLUCURONOSYLTRANSFERASE, BILIRUBIN/PHENOL antibody
    • URIDINE DIPHOSPHATE GLYCOSYLTRANSFERASE 1 antibody
    • URIDINE DIPHOSPHATE GLYCOSYLTRANSFERASE 1 FAMILY, POLYPEPTIDE A1 antibody
    see all

Images

  • Anti-UGT1A1 antibody (ab62600) at 2.5 µg/ml + fetal liver cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 60 kDa
    Observed band size: 60 kDa



    Gel concentration 12%

References

This product has been referenced in:

  • Wang C  et al. Poly(ADP-ribosyl)ated PXR is a critical regulator of acetaminophen-induced hepatotoxicity. Cell Death Dis 9:819 (2018). Read more (PubMed: 30050067) »
  • Zeng H  et al. Schisandrol B protects against cholestatic liver injury through pregnane X receptors. Br J Pharmacol 174:672-688 (2017). Read more (PubMed: 28128437) »
See all 7 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: w/ or w/o 0.01M Sodium Cytrate pH 6.0
Sample
Rat Tissue sections (Liver)
Specification
Liver
Permeabilization
Yes - w/ or w/o 0.1% Triton
Fixative
4% PFA + 30% Sucrose

Abcam user community

Verified customer

Submitted Oct 07 2014

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 20°C
Sample
Rat Tissue sections (Liver)
Specification
Liver
Permeabilization
Yes - w/ or w/o 0.1% triton
Fixative
Acetone or 0.1%Acetic Acid/Ethanol or Methanol

Abcam user community

Verified customer

Submitted Oct 07 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.01M Sodium Cytrate pH 6.0
Sample
Rat Tissue sections (Liver)
Specification
Liver
Permeabilization
Yes - w/ or w/o 0.025% Triton
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Oct 07 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample
Human Cell (ES-derived hepatocytes)
Specification
ES-derived hepatocytes
Permeabilization
Yes - BSA / Tween 0.1%
Fixative
Methanol

Abcam user community

Verified customer

Submitted Sep 10 2013

Application
Western blot
Sample
Mouse Tissue lysate - whole (liver)
Loading amount
50 µg
Specification
liver
Gel Running Conditions
Reduced Denaturing (NuPAGE Novex 4-12% Bis-Tris midi gel (Invitrogen))
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Xia Wen

Verified customer

Submitted Aug 03 2011

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