Anti-UGT1A1 antibody [EPR9592] (ab170858)
- Datasheet
- References (2)
- Protocols
Overview
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Product nameAnti-UGT1A1 antibody [EPR9592]
See all UGT1A1 primary antibodies -
DescriptionRabbit monoclonal [EPR9592] to UGT1A1
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Host speciesRabbit
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Tested applicationsSuitable for: WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivityReacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human UGT1A1 (Cysteine residue). The exact sequence is proprietary.
Database link: P22309 -
Positive control
- Human fetal liver and LoVo lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.16% BSA, 59% PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR9592
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab170858 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Detects a band of approximately 55 kDa (predicted molecular weight: 60 kDa). |
Target
- Information by UniProt
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Database links
- Entrez Gene: 54658 Human
- Omim: 191740 Human
- SwissProt: P22309 Human
- Unigene: 554822 Human
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Alternative names
- BILIQTL1 antibody
- Bilirubin specific UDPGT isozyme 1 antibody
- bilirubin UDP glucuronosyltransferase 1 1 antibody
see all
Images
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![Western blot - Anti-UGT1A1 antibody [EPR9592] (ab170858)](https://www.abcam.com/ps/products/170/ab170858/Images/ab170858-261571-ab170858WB1.jpg)
Western blot - Anti-UGT1A1 antibody [EPR9592] (ab170858)Anti-UGT1A1 antibody [EPR9592] (ab170858) at 1/5000 dilution + LoVo (human colorectal adenocarcinoma) whole cell lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 60 kDa
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.Blocking buffer: 5% NFDM/TBST
Diluting buffer: 5% NFDM/TBST
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![Western blot - Anti-UGT1A1 antibody [EPR9592] (ab170858)](https://www.abcam.com/ps/products/170/ab170858/Images/ab170858-170692-ab1708581.jpg)
Western blot - Anti-UGT1A1 antibody [EPR9592] (ab170858)All lanes : Anti-UGT1A1 antibody [EPR9592] (ab170858) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : LoVo lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 60 kDa
Protocols
Datasheets and documents
References
This product has been referenced in:
- Chen S et al. Intestinal NCoR1, a regulator of epithelial cell maturation, controls neonatal hyperbilirubinemia. Proc Natl Acad Sci U S A 114:E1432-E1440 (2017). Read more (PubMed: 28167773) »
- Chow EC et al. Functional Integrity of the Chimeric (Humanized) Mouse Liver: Enzyme Zonation, Physiologic Spaces, and Hepatic Enzymes and Transporters. Drug Metab Dispos 44:1524-35 (2016). Read more (PubMed: 27342868) »
