Recombinant
RabMAb

Anti-UGT1A1 antibody [EPR9592] (ab170858)

Overview

  • Product name
    Anti-UGT1A1 antibody [EPR9592]
    See all UGT1A1 primary antibodies
  • Description
    Rabbit monoclonal [EPR9592] to UGT1A1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human UGT1A1 (Cysteine residue). The exact sequence is proprietary.
    Database link: P22309

  • Positive control
    • Human fetal liver and LoVo lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab170858 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 55 kDa (predicted molecular weight: 60 kDa).
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Information by UniProt
    • Database links
    • Alternative names
      • BILIQTL1 antibody
      • Bilirubin specific UDPGT isozyme 1 antibody
      • bilirubin UDP glucuronosyltransferase 1 1 antibody
      • bilirubin UDP glucuronosyltransferase isozyme 1 antibody
      • Bilirubin-specific UDPGT isozyme 1 antibody
      • EC 2.4.1.17 antibody
      • GNT1 antibody
      • HUG BR1 antibody
      • HUG-BR1 antibody
      • HUGBR1 antibody
      • PHENOL/BILIRUBIN UDP GLUCURONOSYLTRANSFERASE antibody
      • UD11_HUMAN antibody
      • UDP glucuronosyltransferase 1 1 [Precursor] antibody
      • UDP glucuronosyltransferase 1 family polypeptide A1 antibody
      • UDP glucuronosyltransferase 1A1 antibody
      • UDP GLYCOSYLTRANSFERASE 1 antibody
      • UDP-glucuronosyltransferase 1-1 antibody
      • UDP-glucuronosyltransferase 1-A antibody
      • UDP-glucuronosyltransferase 1A1 antibody
      • UDPGT antibody
      • UDPGT 1-1 antibody
      • UGT 1A antibody
      • UGT-1A antibody
      • UGT1 antibody
      • UGT1 01 antibody
      • UGT1*1 antibody
      • UGT1-01 antibody
      • UGT1.1 antibody
      • UGT1A antibody
      • Ugt1a1 antibody
      • URIDINE DIPHOSPHATE GLUCURONOSYLTRANSFERASE, BILIRUBIN/PHENOL antibody
      • URIDINE DIPHOSPHATE GLYCOSYLTRANSFERASE 1 antibody
      • URIDINE DIPHOSPHATE GLYCOSYLTRANSFERASE 1 FAMILY, POLYPEPTIDE A1 antibody
      see all

    Images

    • Anti-UGT1A1 antibody [EPR9592] (ab170858) at 1/5000 dilution + LoVo (human colorectal adenocarcinoma) whole cell lysate at 20 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size: 60 kDa
      Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.



      Blocking buffer: 5% NFDM/TBST

      Diluting buffer: 5% NFDM/TBST

    • All lanes : Anti-UGT1A1 antibody [EPR9592] (ab170858) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : LoVo lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 60 kDa

    References

    This product has been referenced in:
    • Chen S  et al. Intestinal NCoR1, a regulator of epithelial cell maturation, controls neonatal hyperbilirubinemia. Proc Natl Acad Sci U S A 114:E1432-E1440 (2017). Read more (PubMed: 28167773) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (human cancer cell lines)
    Gel Running Conditions
    Reduced Denaturing (10% resolving, 5% stacking gel)
    Loading amount
    40 µg
    Specification
    human cancer cell lines
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Ms. Joanna Polewska

    Verified customer

    Submitted Dec 19 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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