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RecombinantRabMAb

Recombinant Anti-UGT1A1 antibody [EPR9592] (ab170858)

  • Datasheet
Reviews (1) Submit a question References (4)

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Western blot - Anti-UGT1A1 antibody [EPR9592] (ab170858)
  • Western blot - Anti-UGT1A1 antibody [EPR9592] (ab170858)
  • Anti-UGT1A1 antibody [EPR9592] (ab170858)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9592] to UGT1A1
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-UGT1A1 antibody [EPR9592]
    See all UGT1A1 primary antibodies
  • Description

    Rabbit monoclonal [EPR9592] to UGT1A1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human UGT1A1 (Cysteine residue). The exact sequence is proprietary.
    Database link: P22309

  • Positive control

    • Human fetal liver and LoVo lysates.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.16% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR9592
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Drug metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Drug metabolism

Associated products

  • Alternative Versions

    • Anti-UGT1A1 antibody [EPR9592] - BSA and Azide free (ab240160)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human UGT1A1 protein (ab162807)

Applications

Our Abpromise guarantee covers the use of ab170858 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 55 kDa (predicted molecular weight: 60 kDa).
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Target information above from: UniProt accession P22309 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 54658 Human
      • Omim: 191740 Human
      • SwissProt: P22309 Human
      • Unigene: 554822 Human
      • Alternative names

        • BILIQTL1 antibody
        • Bilirubin specific UDPGT isozyme 1 antibody
        • bilirubin UDP glucuronosyltransferase 1 1 antibody
        • bilirubin UDP glucuronosyltransferase isozyme 1 antibody
        • Bilirubin-specific UDPGT isozyme 1 antibody
        • EC 2.4.1.17 antibody
        • GNT1 antibody
        • HUG BR1 antibody
        • HUG-BR1 antibody
        • HUGBR1 antibody
        • PHENOL/BILIRUBIN UDP GLUCURONOSYLTRANSFERASE antibody
        • UD11_HUMAN antibody
        • UDP glucuronosyltransferase 1 1 [Precursor] antibody
        • UDP glucuronosyltransferase 1 family polypeptide A1 antibody
        • UDP glucuronosyltransferase 1A1 antibody
        • UDP GLYCOSYLTRANSFERASE 1 antibody
        • UDP-glucuronosyltransferase 1-1 antibody
        • UDP-glucuronosyltransferase 1-A antibody
        • UDP-glucuronosyltransferase 1A1 antibody
        • UDPGT antibody
        • UDPGT 1-1 antibody
        • UGT 1A antibody
        • UGT-1A antibody
        • UGT1 antibody
        • UGT1 01 antibody
        • UGT1*1 antibody
        • UGT1-01 antibody
        • UGT1.1 antibody
        • UGT1A antibody
        • Ugt1a1 antibody
        • URIDINE DIPHOSPHATE GLUCURONOSYLTRANSFERASE, BILIRUBIN/PHENOL antibody
        • URIDINE DIPHOSPHATE GLYCOSYLTRANSFERASE 1 antibody
        • URIDINE DIPHOSPHATE GLYCOSYLTRANSFERASE 1 FAMILY, POLYPEPTIDE A1 antibody
        see all

      Images

      • Western blot - Anti-UGT1A1 antibody [EPR9592] (ab170858)
        Western blot - Anti-UGT1A1 antibody [EPR9592] (ab170858)
        Anti-UGT1A1 antibody [EPR9592] (ab170858) at 1/5000 dilution + LoVo (human colorectal adenocarcinoma) whole cell lysate at 20 µg

        Secondary
        Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

        Predicted band size: 60 kDa
        Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.



        Blocking buffer: 5% NFDM/TBST

        Diluting buffer: 5% NFDM/TBST

      • Western blot - Anti-UGT1A1 antibody [EPR9592] (ab170858)
        Western blot - Anti-UGT1A1 antibody [EPR9592] (ab170858)
        All lanes : Anti-UGT1A1 antibody [EPR9592] (ab170858) at 1/1000 dilution

        Lane 1 : Human fetal liver lysate
        Lane 2 : LoVo lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 60 kDa

      • Anti-UGT1A1 antibody [EPR9592] (ab170858)
        Anti-UGT1A1 antibody [EPR9592] (ab170858)

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (4)

      Publishing research using ab170858? Please let us know so that we can cite the reference in this datasheet.

      ab170858 has been referenced in 4 publications.

      • Gu C  et al. The role of insulin in transdifferentiated hepatocyte proliferation and function in serum-free medium. J Cell Mol Med 23:4165-4178 (2019). PubMed: 30950200
      • Nie YL  et al. Histone Modifications Regulate the Developmental Expression of Human Hepatic UDP-Glucuronosyltransferase 1A1. Drug Metab Dispos 45:1372-1378 (2017). PubMed: 29025858
      • Chen S  et al. Intestinal NCoR1, a regulator of epithelial cell maturation, controls neonatal hyperbilirubinemia. Proc Natl Acad Sci U S A 114:E1432-E1440 (2017). PubMed: 28167773
      • Chow EC  et al. Functional Integrity of the Chimeric (Humanized) Mouse Liver: Enzyme Zonation, Physiologic Spaces, and Hepatic Enzymes and Transporters. Drug Metab Dispos 44:1524-35 (2016). PubMed: 27342868

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Western blot abreview for Anti-UGT1A1 antibody [EPR9592]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (human cancer cell lines)
      Gel Running Conditions
      Reduced Denaturing (10% resolving, 5% stacking gel)
      Loading amount
      40 µg
      Specification
      human cancer cell lines
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
      Read More

      Ms. Joanna Polewska

      Verified customer

      Submitted Dec 19 2016

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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