Overview

  • Product name

  • Description

    Rabbit polyclonal to UGT1A6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Amino acids 8-197 of Human UGT1A6 (AAH19861).

  • Positive control

    • 293T, HeLa, HepG2, MOLT4 and Raji cell lines. A431 and H1299 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab97646 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 61 kDa.

Target

  • Function

    UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isoform has specificity for phenols.
  • Tissue specificity

    Expressed in skin, kidney and liver.
  • Sequence similarities

    Belongs to the UDP-glycosyltransferase family.
  • Cellular localization

    Microsome. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • GNT1 antibody
    • HLUGP antibody
    • HLUGP1 antibody
    • Phenol metabolizing UDP glucuronosyltransferase antibody
    • Phenol-metabolizing UDP-glucuronosyltransferase antibody
    • UD16_HUMAN antibody
    • UDP glucuronosyltransferase 1 family polypeptide A6 antibody
    • UDP glucuronosyltransferase 1A6 antibody
    • UDP glycosyltransferase 1 family polypeptide A6 antibody
    • UDP-glucuronosyltransferase 1-6 antibody
    • UDP-glucuronosyltransferase 1-F antibody
    • UDP-glucuronosyltransferase 1A6 antibody
    • UDPGT antibody
    • UDPGT 1-6 antibody
    • UGT 1F antibody
    • UGT-1F antibody
    • UGT1 antibody
    • UGT1 06 antibody
    • UGT1*6 antibody
    • UGT1-06 antibody
    • UGT1.6 antibody
    • UGT1A6 antibody
    • UGT1A6S antibody
    • UGT1F antibody
    see all

Images

  • All lanes : Anti-UGT1A6 antibody (ab97646) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 61 kDa



    7.5% SDS PAGE

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for contacting us. Your credit note ID is xxxxx. I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. The credit note ID is for your reference only and does not automatically guarantee the credit. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

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Answer

Thank you for your enquiry.

I am sorry to confirm that as far as we are aware, ab72598 and ab97646 Cytochrome P450 2C8 + 2C9 + 2C18 + 2C19 antibodies have never been tested with samples from rat. All tested species cross-reactivitycovered by our guaranteeis stated on our datasheets, and these are updated as soon as any new information is brought to our attention.

I have checked the alignment of the rat protein with the immunogen sequences for the antibodies:

ab72598 immunogen ##########
16% alignment to the rat

ab97646 immungoen from a region within amino acids ####
73% alignment to the rat

We recommend that alignment should be over 85% to predict that an antibody will detect in a different species. However, we cannot guarantee this without first testing. As ab97646 has the higher alignment, this would be the preferable antibody to try. At 73% alignment, there may still be some crossreactivity. We would not suggest to try ab72598 in rat as the alignment is so low.

If you would like to test ab97646 in rat, please contact me again by replying to this message prior to the purchase as you may be eligible for our testing discount program.

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact me for any further advice or information in this regard.

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Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Loading amount
50 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing (NuPAGE Novex 4-12% Bis-Tris mini gel from Invitrogen)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Xia Wen

Verified customer

Submitted Jul 25 2011

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