Recombinant Anti-UGT1A6 antibody [EPR11068] (ab157476)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11068] to UGT1A6
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-UGT1A6 antibody [EPR11068]
See all UGT1A6 primary antibodies -
Description
Rabbit monoclonal [EPR11068] to UGT1A6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human UGT1A6 aa 400-500 (Cysteine residue). The exact sequence is proprietary.
Database link: P19224 -
Positive control
- Caco-2, fetal liver and A431 lysates; Human colon and liver tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11068 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab157476 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/1000 - 1/10000. Predicted molecular weight: 61 kDa. | |
IHC-P | 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isoform has specificity for phenols. -
Tissue specificity
Expressed in skin, kidney and liver. -
Sequence similarities
Belongs to the UDP-glycosyltransferase family. -
Cellular localization
Microsome. Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 54578 Human
- Entrez Gene: 94284 Mouse
- Entrez Gene: 113992 Rat
- Omim: 606431 Human
- SwissProt: P19224 Human
- SwissProt: Q64435 Mouse
- SwissProt: P08430 Rat
- Unigene: 554822 Human
see all -
Alternative names
- GNT1 antibody
- HLUGP antibody
- HLUGP1 antibody
see all
Images
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All lanes : Anti-UGT1A6 antibody [EPR11068] (ab157476) at 1/1000 dilution
Lane 1 : Caco-2 cell lysate
Lane 2 : Fetal liver lysate
Lane 3 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 61 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGT1A6 antibody [EPR11068] (ab157476)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling UGT1A6 with ab157476 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGT1A6 antibody [EPR11068] (ab157476)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling UGT1A6 with ab157476 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
References (0)
ab157476 has not yet been referenced specifically in any publications.