Product nameAnti-UGT2B4 antibody [EPR11677]
DescriptionRabbit monoclonal [EPR11677] to UGT2B4
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: FM,ICC/IF or IHC-P
Species reactivityReacts with: Human
- Human Fetal liver, HepG2, LNCap lysates. HepG2 cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab173580 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/100000. Predicted molecular weight: 61 kDa.|
|IP||1/10 - 1/100.|
RelevanceThe UDP-Glucuronosyltransferases (UGT) comprise a family of enzymes that detoxify and enhance the urinary excretion of a wide variety of xenobiotic and endogenous substrates by transferring glucuronic acid to sulfhydryl, hydroxyl, aromatic amino, or carboxylic acid groups. They have been subdivided into two families, UGT1 and UGT2, based on the evolutionary divergence of their genes. The enzymes of the UGT1A family play an important role in the metabolism of dietary constituents, phenols, and therapeutic drugs, and also the glucuronidation of bilirubin and iodothyronines. The enzymes of the UGT2B family are involved in the metabolism of bile acids, phenol derivatives, catecholestrogens and steroids. Although it is widely recognized that the liver is the major site of glucuronidation, it is now clear that UGT enzymes are also found in extra-hepatic tissues.
- EC 18.104.22.168 antibody
- HLUG25 antibody
- Hyodeoxycholic acid antibody
Western blot analysis on immunoprecipitation pellet from (1) fetal liver lysate or (2) 1X PBS (negative control) using ab173580 at 1/10 dilution immunoprecipitating UGT2B4, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
All lanes : Anti-UGT2B4 antibody [EPR11677] (ab173580) at 1/1000 dilution
Lane 1 : Human fetal liver cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : LNCap cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 61 kDa
This product has been referenced in:
- Chen X et al. SPIN1, negatively regulated by miR-148/152, enhances Adriamycin resistance via upregulating drug metabolizing enzymes and transporter in breast cancer. J Exp Clin Cancer Res 37:100 (2018). WB . Read more (PubMed: 29743122) »