Overview

  • Product name

    Anti-UGT3A1 antibody [EPR13723]
    See all UGT3A1 primary antibodies
  • Description

    Rabbit monoclonal [EPR13723] to UGT3A1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human UGT3A1 aa 1-150. The exact sequence is proprietary.
    Database link: Q6NUS8

  • Positive control

    • Human testis, fetal liver and fetal kidney cell lysates; HepG2 cell lysate; Human liver and kidney tissues; HepG2 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181245 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/500.
IP 1/40 - 1/60.

Target

  • Function

    UDP-glucuronosyltransferases catalyze phase II biotransformation reactions in which lipophilic substrates are conjugated with glucuronic acid to increase water solubility and enhance excretion. They are of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds.
  • Sequence similarities

    Belongs to the UDP-glycosyltransferase family.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ34658 antibody
    • UD3A1_HUMAN antibody
    • UDP glycosyltransferase 3 family, polypeptide A1 antibody
    • UDP-glucuronosyltransferase 3A1 antibody
    • UDPGT 3A1 antibody
    • UGT3A 1 antibody
    • Ugt3a1 antibody
    see all

Images

  • All lanes : Anti-UGT3A1 antibody [EPR13723] (ab181245) at 1/1000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Human fetal kidney lysate
    Lane 3 : Human testis lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Developed using the ECL technique.

    Predicted band size: 59 kDa
    Observed band size: 59 kDa

  • Immunofluorescence analysis of, paraformaldehyde-fixed, HepG2 cells labeling UGT3A1 with ab181245 at a 1/500 dilution. As secondary antibody goat anti rabbit IgG (Alexa Fluor®555) was used at a 1/200. In blue DAPI staining.

  • Anti-UGT3A1 antibody [EPR13723] (ab181245) at 1/1000 dilution + HepG2 cell lysate at 10 µg

    Secondary
    goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 59 kDa
    Observed band size: 59 kDa

  • Immunohistochemical analysis of paraffin-embedded, Human liver tissue labeling UGT3A1 with ab181245 at a 1/100 dilution. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded, Human kidney tissue labeling UGT3A1 with ab181245 at a 1/100 dilution. Counter stained with hematoxylin.

  • Western blot analysis on immunoprecipitation from 1) Human fetal liver lysate or 2) negative control, labeling UGT3A1 using ab181245 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.

References

ab181245 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab181245.
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