Anti-UHRF1 antibody (ab126243)
Key features and details
- Rabbit polyclonal to UHRF1
- Suitable for: IHC-P, ChIP, WB, ICC/IF, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-UHRF1 antibody
See all UHRF1 primary antibodies -
Description
Rabbit polyclonal to UHRF1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ChIP, WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to a region within amino acids 503 and 733 of Human UHRF1 (AAI13876)
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Positive control
- ChIP: HCT 116 chromatin extract. WB: HCT116 whole cell lysate. IHC-P: HBL435 xenograft tissue. IF: A549 and HeLa cells. IP: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126243 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/1000.
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ChIP |
Use at an assay dependent concentration.
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 51 kDa.
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ICC/IF |
1/100 - 1/1000.
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IP |
1/100 - 1/500.
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Notes |
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IHC-P
1/100 - 1/1000. |
ChIP
Use at an assay dependent concentration. |
WB
1/500 - 1/3000. Predicted molecular weight: 51 kDa. |
ICC/IF
1/100 - 1/1000. |
IP
1/100 - 1/500. |
Target
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Function
Putative E3 ubiquitin-protein ligase. May participate in methylation-dependent transcriptional regulation. Binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. Important for G1/S transition. May be involved in DNA repair and chromosomal stability. -
Tissue specificity
Expressed in thymus, bone marrow, testis, lung and heart. Overexpressed in breast cancer. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 PHD-type zinc finger.
Contains 2 RING-type zinc fingers.
Contains 1 ubiquitin-like domain.
Contains 1 YDG domain. -
Developmental stage
Expressed in fetal thymus, liver and kidney. -
Domain
The RING finger is required for ubiquitin ligase activity.
The YDG domain mediates the interaction with histone H3. -
Post-translational
modificationsPhosphorylated on serine residues. Phosphorylation may enhance DNA-binding activity.
Ubiquitinated; which leads to proteasomal degradation. Polyubiquitination may be stimulated by DNA damage. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 29128 Human
- Omim: 607990 Human
- SwissProt: Q96T88 Human
- Unigene: 108106 Human
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Alternative names
- Ac2-121 antibody
- AL022808 antibody
- E3 ubiquitin-protein ligase UHRF1 antibody
see all
Images
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Cross-linked ChIP was performed with HCT 116 (human colorectal carcinoma cell line) chromatin extract and 5 μg of either control rabbit IgG or ab126243. The precipitated DNA was detected by PCR with primer set targeting to PPARG promoter.
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Anti-UHRF1 antibody (ab126243) at 1/1000 dilution + HCT116 (human colorectal carcinoma cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 51 kDa
7.5% SDS PAGE -
Paraffin-embedded HBL435 xenograft tissue stained UHRF1 using ab126243 at 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
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Immunofluorescence analysis of 4% paraformaldehyde fixed (RT for 15 min) A549 (human lung carcinoma cell line) whole cell labelling UHRF1 in the nucleus (green) at 1/250. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at 1/1000 dilution.
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IP Sample: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate.
1 - 40 μg HeLa whole cell lysate.
2 - Control with 2 μg of preimmune rabbit IgG.
3 - Immunoprecipitation of UHRF1 protein by 2 μg of ab126243.
5% SDS-PAGE
The immunoprecipitated UHRF1 protein was detected by ab126243 at 1/1000 dilution, followed by anti-rabbit IgG antibody.
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Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling UHRF1 using ab126243 (green) at 1/500 dilution. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at 1/2000 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab126243 has not yet been referenced specifically in any publications.