Anti-UHRF1 antibody (ab153972)

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Western blot - Anti-UHRF1 antibody (ab153972)
  • Immunocytochemistry/ Immunofluorescence - Anti-UHRF1 antibody (ab153972)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody (ab153972)

Key features and details

  • Rabbit polyclonal to UHRF1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-UHRF1 antibody
    See all UHRF1 primary antibodies

  • Description

    Rabbit polyclonal to UHRF1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 51-516 of Human UHRF1.

  • Positive control

    • HeLa whole cell lysate; Human BT483 xenograft; HeLa cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab153972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 89 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 89 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF
1/100 - 1/1000.

Target

  • Function

    Putative E3 ubiquitin-protein ligase. May participate in methylation-dependent transcriptional regulation. Binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. Important for G1/S transition. May be involved in DNA repair and chromosomal stability.

  • Tissue specificity

    Expressed in thymus, bone marrow, testis, lung and heart. Overexpressed in breast cancer.

  • Pathway

    Protein modification; protein ubiquitination.

  • Sequence similarities

    Contains 1 PHD-type zinc finger.
    Contains 2 RING-type zinc fingers.
    Contains 1 ubiquitin-like domain.
    Contains 1 YDG domain.

  • Developmental stage

    Expressed in fetal thymus, liver and kidney.

  • Domain

    The RING finger is required for ubiquitin ligase activity.
    The YDG domain mediates the interaction with histone H3.

  • Post-translational
    modifications

    Phosphorylated on serine residues. Phosphorylation may enhance DNA-binding activity.
    Ubiquitinated; which leads to proteasomal degradation. Polyubiquitination may be stimulated by DNA damage.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • Ac2-121 antibody
      • AL022808 antibody
      • E3 ubiquitin-protein ligase UHRF1 antibody
      • EC 6.3.2.- antibody
      • FLJ21925 antibody
      • hNP95 antibody
      • hUHRF1 antibody
      • HuNp95 antibody
      • ICBP90 antibody
      • Inverted CCAAT box binding protein of 90 kDa antibody
      • Inverted CCAAT box binding protein, 90-kD antibody
      • Inverted CCAAT box-binding protein of 90 kDa antibody
      • Liver regeneration-related protein LRRG126 antibody
      • MGC138707 antibody
      • NP95 antibody
      • Nuclear phosphoprotein 95 antibody
      • Nuclear phosphoprotein, 95-KD antibody
      • Nuclear protein 95 antibody
      • Nuclear zinc finger protein Np95 antibody
      • RING finger protein 106 antibody
      • RNF106 antibody
      • TDRD22 antibody
      • Transcription factor ICBP90 antibody
      • Ubiquitin like containing PHD and RING finger domains protein 1 antibody
      • Ubiquitin like PHD and RING finger domain containing protein 1 antibody
      • Ubiquitin-like PHD and RING finger domain-containing protein 1 antibody
      • Ubiquitin-like protein containing PHD and RING finger domains 1 antibody
      • Ubiquitin-like with PHD and ring finger domains 1 antibody
      • Ubiquitin-like, containing PHD and RING finger domains, 1 antibody
      • Ubiquitin-like-containing PHD and RING finger domains protein 1 antibody
      • UHRF1 antibody
      • UHRF1_HUMAN antibody
      see all

    Images

    • Western blot - Anti-UHRF1 antibody (ab153972)
      Western blot - Anti-UHRF1 antibody (ab153972)
      Anti-UHRF1 antibody (ab153972) at 1/2000 dilution + HeLa whole cell lysate at 30 µg

      Predicted band size: 89 kDa



      7.5% SDS PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-UHRF1 antibody (ab153972)
      Immunocytochemistry/ Immunofluorescence - Anti-UHRF1 antibody (ab153972)
      Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling UHRF1 with ab153972 at 1/500 dilution (Green). Alpha-tubulin filaments were labeled Red.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody (ab153972)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody (ab153972)
      Immunohistochemical analysis of paraffin-embedded Human BT483 xenograft labeling UHRF1 with ab153972 at 1/250 dilution.

    References (1)

    Publishing research using ab153972? Please let us know so that we can cite the reference in this datasheet.

    ab153972 has been referenced in 1 publication.

    • Antonova A  et al. Heat-Shock Protein 90 Controls the Expression of Cell-Cycle Genes by Stabilizing Metazoan-Specific Host-Cell Factor HCFC1. Cell Rep 29:1645-1659.e9 (2019). PubMed: 31693902

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