Anti-UHRF1 antibody (ab153972)

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Overview

  • Product name

    Anti-UHRF1 antibody
    See all UHRF1 primary antibodies

  • Description

    Rabbit polyclonal to UHRF1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 51-516 of Human UHRF1.

  • Positive control

    • HeLa whole cell lysate; Human BT483 xenograft; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab153972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 89 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Putative E3 ubiquitin-protein ligase. May participate in methylation-dependent transcriptional regulation. Binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. Important for G1/S transition. May be involved in DNA repair and chromosomal stability.

  • Tissue specificity

    Expressed in thymus, bone marrow, testis, lung and heart. Overexpressed in breast cancer.

  • Pathway

    Protein modification; protein ubiquitination.

  • Sequence similarities

    Contains 1 PHD-type zinc finger.
    Contains 2 RING-type zinc fingers.
    Contains 1 ubiquitin-like domain.
    Contains 1 YDG domain.

  • Developmental stage

    Expressed in fetal thymus, liver and kidney.

  • Domain

    The RING finger is required for ubiquitin ligase activity.
    The YDG domain mediates the interaction with histone H3.

  • Post-translational
    modifications

    Phosphorylated on serine residues. Phosphorylation may enhance DNA-binding activity.
    Ubiquitinated; which leads to proteasomal degradation. Polyubiquitination may be stimulated by DNA damage.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • Ac2-121 antibody
      • AL022808 antibody
      • E3 ubiquitin-protein ligase UHRF1 antibody
      • EC 6.3.2.- antibody
      • FLJ21925 antibody
      • hNP95 antibody
      • hUHRF1 antibody
      • HuNp95 antibody
      • ICBP90 antibody
      • Inverted CCAAT box binding protein of 90 kDa antibody
      • Inverted CCAAT box binding protein, 90-kD antibody
      • Inverted CCAAT box-binding protein of 90 kDa antibody
      • Liver regeneration-related protein LRRG126 antibody
      • MGC138707 antibody
      • NP95 antibody
      • Nuclear phosphoprotein 95 antibody
      • Nuclear phosphoprotein, 95-KD antibody
      • Nuclear protein 95 antibody
      • Nuclear zinc finger protein Np95 antibody
      • RING finger protein 106 antibody
      • RNF106 antibody
      • TDRD22 antibody
      • Transcription factor ICBP90 antibody
      • Ubiquitin like containing PHD and RING finger domains protein 1 antibody
      • Ubiquitin like PHD and RING finger domain containing protein 1 antibody
      • Ubiquitin-like PHD and RING finger domain-containing protein 1 antibody
      • Ubiquitin-like protein containing PHD and RING finger domains 1 antibody
      • Ubiquitin-like with PHD and ring finger domains 1 antibody
      • Ubiquitin-like, containing PHD and RING finger domains, 1 antibody
      • Ubiquitin-like-containing PHD and RING finger domains protein 1 antibody
      • UHRF1 antibody
      • UHRF1_HUMAN antibody
      see all

    Images

    • Western blot - Anti-UHRF1 antibody (ab153972)
      Western blot - Anti-UHRF1 antibody (ab153972)
      Anti-UHRF1 antibody (ab153972) at 1/2000 dilution + HeLa whole cell lysate at 30 µg

      Predicted band size: 89 kDa



      7.5% SDS PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-UHRF1 antibody (ab153972)
      Immunocytochemistry/ Immunofluorescence - Anti-UHRF1 antibody (ab153972)
      Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling UHRF1 with ab153972 at 1/500 dilution (Green). Alpha-tubulin filaments were labeled Red.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody (ab153972)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody (ab153972)
      Immunohistochemical analysis of paraffin-embedded Human BT483 xenograft labeling UHRF1 with ab153972 at 1/250 dilution.

    References

    ab153972 has not yet been referenced specifically in any publications.

    Publishing research using ab153972? Please let us know so that we can cite the reference in this datasheet.

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