Overview

  • Product name

    Anti-UHRF1 antibody [EPR18803-11]
    See all UHRF1 primary antibodies
  • Description

    Rabbit monoclonal [EPR18803-11] to UHRF1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human UHRF1 aa 1-200. The exact sequence is proprietary.
    Database link: Q96T88

  • Positive control

    • WB: Human colon and spleen lysates; HeLa, Jurkat, HepG2. IHC-P: Human tonsil and colon cancer tissues. ICC/IF: Jurkat and HeLa cells. Flow Cyt: Jurkat cells. IP: HeLa whole cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab213223 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 97 kDa (predicted molecular weight: 89 kDa).
IHC-P 1/100.

Recommended for human only

ICC/IF 1/500.

Recommended for human only

Flow Cyt 1/70.
IP 1/30.

Target

  • Function

    Putative E3 ubiquitin-protein ligase. May participate in methylation-dependent transcriptional regulation. Binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. Important for G1/S transition. May be involved in DNA repair and chromosomal stability.
  • Tissue specificity

    Expressed in thymus, bone marrow, testis, lung and heart. Overexpressed in breast cancer.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 1 PHD-type zinc finger.
    Contains 2 RING-type zinc fingers.
    Contains 1 ubiquitin-like domain.
    Contains 1 YDG domain.
  • Developmental stage

    Expressed in fetal thymus, liver and kidney.
  • Domain

    The RING finger is required for ubiquitin ligase activity.
    The YDG domain mediates the interaction with histone H3.
  • Post-translational
    modifications

    Phosphorylated on serine residues. Phosphorylation may enhance DNA-binding activity.
    Ubiquitinated; which leads to proteasomal degradation. Polyubiquitination may be stimulated by DNA damage.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Ac2-121 antibody
    • AL022808 antibody
    • E3 ubiquitin-protein ligase UHRF1 antibody
    • EC 6.3.2.- antibody
    • FLJ21925 antibody
    • hNP95 antibody
    • hUHRF1 antibody
    • HuNp95 antibody
    • ICBP90 antibody
    • Inverted CCAAT box binding protein of 90 kDa antibody
    • Inverted CCAAT box binding protein, 90-kD antibody
    • Inverted CCAAT box-binding protein of 90 kDa antibody
    • Liver regeneration-related protein LRRG126 antibody
    • MGC138707 antibody
    • NP95 antibody
    • Nuclear phosphoprotein 95 antibody
    • Nuclear phosphoprotein, 95-KD antibody
    • Nuclear protein 95 antibody
    • Nuclear zinc finger protein Np95 antibody
    • RING finger protein 106 antibody
    • RNF106 antibody
    • TDRD22 antibody
    • Transcription factor ICBP90 antibody
    • Ubiquitin like containing PHD and RING finger domains protein 1 antibody
    • Ubiquitin like PHD and RING finger domain containing protein 1 antibody
    • Ubiquitin-like PHD and RING finger domain-containing protein 1 antibody
    • Ubiquitin-like protein containing PHD and RING finger domains 1 antibody
    • Ubiquitin-like with PHD and ring finger domains 1 antibody
    • Ubiquitin-like, containing PHD and RING finger domains, 1 antibody
    • Ubiquitin-like-containing PHD and RING finger domains protein 1 antibody
    • UHRF1 antibody
    • UHRF1_HUMAN antibody
    see all

Images

  • All lanes : Anti-UHRF1 antibody [EPR18803-11] (ab213223) at 1/1000 dilution

    Lane 1 : Human colon lysate
    Lane 2 : Human spleen lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

    Predicted band size: 89 kDa
    Observed band size: 97 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-UHRF1 antibody [EPR18803-11] (ab213223) at 1/5000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 89 kDa
    Observed band size: 97 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1 and 2:5 seconds; Lane 3: 30 seconds.

  • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling UHRF1 with ab213223 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Nucleus staining on Human tonsil germinal center is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling UHRF1 with ab213223 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Nucleus staining on Human colon cancer is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling UHRF1 with ab213223 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor®488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing nuclear staining on HeLa cell line. 

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (Goat anti-Mouse IgG (Alexa Fluor® 594)) at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab213223 at 1/500 dilution followed by ab150120 at 1/1000 dilution.

    -ve control 2: ab7291  at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling UHRF1 with ab213223 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor®488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing nuclear staining on HeLa cell line. 

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (Goat anti-Mouse IgG (Alexa Fluor® 594)) at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab213223 at 1/500 dilution followed by ab150120 at 1/1000 dilution.

    -ve control 2: ab7291  at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

  • Flow cytometric analysis of 4% paraformaldehyde-fixed Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling UHRF1 with ab213223 at 1/70 dilution (red) compared with a Rabbit IgG,monoclonal [EPR25A] - Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

  • UHRF1 was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab213223 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab213223 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

    Lane 1: HeLa whole cell lysate, 10µg (Input).

    Lane 2: ab213223 IP in HeLa whole cell lysate.

    Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab213223 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

References

This product has been referenced in:

  • Chen X  et al. Overexpression of UHRF1 promoted the proliferation of vascular smooth cells via the regulation of Geminin protein levels. Biosci Rep 39:N/A (2019). Read more (PubMed: 30710064) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (Human ES Cells)
Gel Running Conditions
Reduced Denaturing (4-14% Nupage Bis-Tris)
Loading amount
308000 cells
Specification
Human ES Cells
Blocking step
Li-Cor Odyssey PBS Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Mr. Timothy Hunt

Verified customer

Submitted Sep 12 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Cell (Gonad)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9
Permeabilization
Yes - 0.05% triton X in PBS
Specification
Gonad
Blocking step
No blocking step used for 30 hour(s) and 0 minute(s) · Concentration: 10 · Temperature: 25°C
Fixative
Paraformaldehyde

Mr. Timothy Hunt

Verified customer

Submitted Aug 23 2017

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