RecombinantRabMAb

Recombinant Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388)
  • Immunocytochemistry/ Immunofluorescence - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388)
  • Immunocytochemistry/ Immunofluorescence - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388)
  • Flow Cytometry - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388)
  • Immunoprecipitation - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388)
  • Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18803-11] to UHRF1 - BSA and Azide free
  • Suitable for: Flow Cyt, IHC-P, WB, ICC/IF, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free
    See all UHRF1 primary antibodies

  • Description

    Rabbit monoclonal [EPR18803-11] to UHRF1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WB, ICC/IF, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human UHRF1 aa 1-200. The exact sequence is proprietary.
    Database link: Q96T88

  • General notes

    Ab240388 is the carrier-free version of ab213223. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240388 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab240388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Recommended for human only.
WB Use at an assay dependent concentration. Detects a band of approximately 97 kDa (predicted molecular weight: 89 kDa).
ICC/IF Use at an assay dependent concentration.

Recommended for human only.
IP Use at an assay dependent concentration.

Target

  • Function

    Putative E3 ubiquitin-protein ligase. May participate in methylation-dependent transcriptional regulation. Binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. Important for G1/S transition. May be involved in DNA repair and chromosomal stability.

  • Tissue specificity

    Expressed in thymus, bone marrow, testis, lung and heart. Overexpressed in breast cancer.

  • Pathway

    Protein modification; protein ubiquitination.

  • Sequence similarities

    Contains 1 PHD-type zinc finger.
    Contains 2 RING-type zinc fingers.
    Contains 1 ubiquitin-like domain.
    Contains 1 YDG domain.

  • Developmental stage

    Expressed in fetal thymus, liver and kidney.

  • Domain

    The RING finger is required for ubiquitin ligase activity.
    The YDG domain mediates the interaction with histone H3.

  • Post-translational
    modifications

    Phosphorylated on serine residues. Phosphorylation may enhance DNA-binding activity.
    Ubiquitinated; which leads to proteasomal degradation. Polyubiquitination may be stimulated by DNA damage.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • Ac2-121 antibody
      • AL022808 antibody
      • E3 ubiquitin-protein ligase UHRF1 antibody
      • EC 6.3.2.- antibody
      • FLJ21925 antibody
      • hNP95 antibody
      • hUHRF1 antibody
      • HuNp95 antibody
      • ICBP90 antibody
      • Inverted CCAAT box binding protein of 90 kDa antibody
      • Inverted CCAAT box binding protein, 90-kD antibody
      • Inverted CCAAT box-binding protein of 90 kDa antibody
      • Liver regeneration-related protein LRRG126 antibody
      • MGC138707 antibody
      • NP95 antibody
      • Nuclear phosphoprotein 95 antibody
      • Nuclear phosphoprotein, 95-KD antibody
      • Nuclear protein 95 antibody
      • Nuclear zinc finger protein Np95 antibody
      • RING finger protein 106 antibody
      • RNF106 antibody
      • TDRD22 antibody
      • Transcription factor ICBP90 antibody
      • Ubiquitin like containing PHD and RING finger domains protein 1 antibody
      • Ubiquitin like PHD and RING finger domain containing protein 1 antibody
      • Ubiquitin-like PHD and RING finger domain-containing protein 1 antibody
      • Ubiquitin-like protein containing PHD and RING finger domains 1 antibody
      • Ubiquitin-like with PHD and ring finger domains 1 antibody
      • Ubiquitin-like, containing PHD and RING finger domains, 1 antibody
      • Ubiquitin-like-containing PHD and RING finger domains protein 1 antibody
      • UHRF1 antibody
      • UHRF1_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388)

      Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling UHRF1 with ab213223 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Nucleus staining on Human tonsil germinal center is observed.

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213223).

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388)

      Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling UHRF1 with ab213223 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Nucleus staining on Human colon cancer is observed.

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213223).

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388)
      Immunocytochemistry/ Immunofluorescence - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling UHRF1 with ab213223 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor®488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Confocal image showing nuclear staining on HeLa cell line. 

      The nuclear counterstain is DAPI (blue).

      Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (Goat anti-Mouse IgG (Alexa Fluor® 594)) at 1/1000 dilution (red).

      The negative controls are as follows:-

      -ve control 1: ab213223 at 1/500 dilution followed by ab150120 at 1/1000 dilution.

      -ve control 2: ab7291  at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213223).

    • Immunocytochemistry/ Immunofluorescence - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388)
      Immunocytochemistry/ Immunofluorescence - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling UHRF1 with ab213223 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor®488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Confocal image showing nuclear staining on HeLa cell line. 

      The nuclear counterstain is DAPI (blue).

      Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (Goat anti-Mouse IgG (Alexa Fluor® 594)) at 1/1000 dilution (red).

      The negative controls are as follows:-

      -ve control 1: ab213223 at 1/500 dilution followed by ab150120 at 1/1000 dilution.

      -ve control 2: ab7291  at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213223).

    • Flow Cytometry - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388)
      Flow Cytometry - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388)

      Flow cytometric analysis of 4% paraformaldehyde-fixed Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling UHRF1 with ab213223 at 1/70 dilution (red) compared with a Rabbit IgG,monoclonal [EPR25A] - Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213223).

    • Immunoprecipitation - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388)
      Immunoprecipitation - Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388)

      UHRF1 was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab213223 at 1/30 dilution.

      Western blot was performed from the immunoprecipitate using ab213223 at 1/1000 dilution.

      VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

      Lane 1: HeLa whole cell lysate, 10µg (Input).

      Lane 2: ab213223 IP in HeLa whole cell lysate.

      Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab213223 in HeLa whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 1 second.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213223).

    • Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388)
      Anti-UHRF1 antibody [EPR18803-11] - BSA and Azide free (ab240388)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab240388? Please let us know so that we can cite the reference in this datasheet.

    ab240388 has not yet been referenced specifically in any publications.

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