Recombinant
RabMAb

Recombinant Anti-ULBP1 antibody [EPR22088-211] (ab224711)

Overview

  • Product name

    Anti-ULBP1 antibody [EPR22088-211]
    See all ULBP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR22088-211] to ULBP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P,IP or WB
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human ULBP1 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9BZM6

  • Positive control

    • Flow cyt: MOLT-4 cells; human peripheral blood mononuclear cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22088-211
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab224711 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/60.
  • Application notes
    Is unsuitable for ICC/IF,IHC-P,IP or WB.
  • Target

    • Function

      Ligand for the NKG2D receptor, together with at least ULBP2 and ULBP3. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. In CMV infected cells, interacts with soluble CMV glycoprotein UL16. The interaction with UL16 blocked the interaction with the NKG2D receptor, providing a mechanism by which CMV infected cells might escape the immune system. UL16 also causes ULBP1 to be retained in the ER and cis-Golgi apparatus so that it does not reach the cell surface.
    • Tissue specificity

      Expressed in T-cells, B-cells, erythroleukemia cell lines and in a wide range of tissues including heart, brain, lung, liver, testis, lymph node, thymus, tonsil and bone marrow. Also found in fetal heart, brain, lung and liver.
    • Sequence similarities

      Belongs to the MHC class I family.
    • Cellular localization

      Cell membrane. Endoplasmic reticulum. Detected intracellularly in the endoplasmic reticulum of CMV-infected fibroblasts.
    • Information by UniProt
    • Database links

    • Alternative names

      • Alcan beta antibody
      • ALCAN-beta antibody
      • N2DL-1 antibody
      • N2DL1 antibody
      • N2DL1_HUMAN antibody
      • NKG2D ligand 1 antibody
      • NKG2D ligand 1 precursor antibody
      • NKG2DL1 antibody
      • RAET1I antibody
      • Retinoic acid early transcript 1I antibody
      • UL16 antibody
      • UL16 binding protein 1 antibody
      • UL16-binding protein 1 antibody
      • ULBP1 antibody
      see all

    Images

    • Flow cytometric analysis of Human peripheral blood mononuclear (PBMC) cells labelling ULBP1 with ab224711 at 1/60 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730, Left) isotype control. Goat anti rabbit IgG (Alexa Fluor® 488, ab150097) at 1/5000 dilution was used as the secondary antibody.

      Cells were stained with rabbit IgG (Left) or ab224711 (Right). Then stained with anti-CD14 conjugated to BV510.  Gated on viable cells.

    • Flow cytometric analysis of HaCaT (Human skin keratinocyte, Left) / MOLT-4 (Human lymphoblastic leukemia T lymphoblast, Right) cells labelling ULBP1 with ab224711 at 1/60 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

      Negative control: HaCaT. (PMID: 22316211) Gated on viable cells.

    References

    ab224711 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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