Overview

  • Product name
    Anti-ULBP1 antibody [EPR7532(2)]
    See all ULBP1 primary antibodies
  • Description
    Rabbit monoclonal [EPR7532(2)] to ULBP1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human ULBP1 aa 1-100. The exact sequence is proprietary.
    Database link: Q9BZM6

  • Positive control
    • Lysate from CHO cells transfected with ULBP1; HeLa, K562 and 293T cell lysates; K562 cells.
  • General notes

    This antibody was developed as part of a collaboration project between Epitomics and Hugh Reyburn at the Centro Nacional de Biotechnologia, Madrid.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR7532(2)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab176566 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function
      Ligand for the NKG2D receptor, together with at least ULBP2 and ULBP3. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. In CMV infected cells, interacts with soluble CMV glycoprotein UL16. The interaction with UL16 blocked the interaction with the NKG2D receptor, providing a mechanism by which CMV infected cells might escape the immune system. UL16 also causes ULBP1 to be retained in the ER and cis-Golgi apparatus so that it does not reach the cell surface.
    • Tissue specificity
      Expressed in T-cells, B-cells, erythroleukemia cell lines and in a wide range of tissues including heart, brain, lung, liver, testis, lymph node, thymus, tonsil and bone marrow. Also found in fetal heart, brain, lung and liver.
    • Sequence similarities
      Belongs to the MHC class I family.
    • Cellular localization
      Cell membrane. Endoplasmic reticulum. Detected intracellularly in the endoplasmic reticulum of CMV-infected fibroblasts.
    • Information by UniProt
    • Database links
    • Alternative names
      • Alcan beta antibody
      • ALCAN-beta antibody
      • N2DL-1 antibody
      • N2DL1 antibody
      • N2DL1_HUMAN antibody
      • NKG2D ligand 1 antibody
      • NKG2D ligand 1 precursor antibody
      • NKG2DL1 antibody
      • RAET1I antibody
      • Retinoic acid early transcript 1I antibody
      • UL16 antibody
      • UL16 binding protein 1 antibody
      • UL16-binding protein 1 antibody
      • ULBP1 antibody
      see all

    Images

    • All lanes : Anti-ULBP1 antibody [EPR7532(2)] (ab176566) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 28 kDa

    • Flow cytometry analysis of permeabilized K562 cells labeling ULBP1 (red) with ab176566 at a 1/100 dilution, or negative control rabbit IgG (green)

    • All lanes : Anti-ULBP1 antibody [EPR7532(2)] (ab176566) at 1/1000 dilution

      Lane 1 : Lysate from CHO cells mock-transfected
      Lane 2 : Lysate from CHO cells transfected with ULBP1

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 28 kDa

    References

    This product has been referenced in:
    • Campbell TM  et al. Varicella-Zoster Virus and Herpes Simplex Virus 1 Differentially Modulate NKG2D Ligand Expression during Productive Infection. J Virol 89:7932-43 (2015). Read more (PubMed: 25995251) »

    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab176566.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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