Key features and details
- Mouse polyclonal to ULBP2
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ULBP2 antibody
See all ULBP2 primary antibodies
DescriptionMouse polyclonal to ULBP2
Tested applicationsSuitable for: WB, Flow Cytmore details
Species reactivityReacts with: Human
Full length protein corresponding to Human ULBP2 aa 1-246.
Database link: Q9BZM5
- Human ovary cancer tissue lysate. Cell lysate from transfected 293T cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab88645 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 27 kDa.|
|Flow Cyt||Use at an assay dependent concentration.
ab37355 - Mouse polyclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionLigand for the NKG2D receptor, together with at least ULBP1 and ULBP3. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. In CMV infected cells, interacts with soluble CMV glycoprotein UL16. The interaction with UL16 blocked the interaction with the NKG2D receptor, providing a mechanism by which CMV infected cells might escape the immune system. UL16 also causes ULBP2 to be retained in the ER and cis-Golgi apparatus so that it does not reach the cell surface.
Tissue specificityExpressed in various types of cancer cell lines and in the fetus, but not in normal tissues.
Sequence similaritiesBelongs to the MHC class I family.
Cellular localizationCell membrane. Secreted.
- Information by UniProt
- ALCAN alpha antibody
- ALCAN-alpha antibody
- N2DL 2 antibody
ab88645 detecting ULBP2 in ULBP2-expressing 293 cells (Green) compared with negative control 293 cells (Black) by flow cytometry.
Anti-ULBP2 antibody (ab88645) at 1 µg/ml + Human ovary cancer tissue lysate at 50 µg
Predicted band size: 27 kDa
Observed band size: ~35 kDa why is the actual band size different from the predicted?
All lanes : Anti-ULBP2 antibody (ab88645) at 1 µg/ml
Lane 1 : cell lysate from ULBP2 transfected 293T cells
Lane 2 : cell lysate from non transfected 293T cells
Lysates/proteins at 25 µg per lane.
Predicted band size: 27 kDa
Observed band size: ~29 kDa why is the actual band size different from the predicted?
Additional bands at: ~27 kDa, ~33 kDa. We are unsure as to the identity of these extra bands.
ab88645 has been referenced in 1 publication.
- Dudek-Peric AM et al. Antitumor immunity triggered by melphalan is potentiated by melanoma cell surface-associated calreticulin. Cancer Res 75:1603-14 (2015). WB ; Human . PubMed: 25762540