Overview

  • Product name

    Anti-ULBP3 antibody [MM0594-6E12]
    See all ULBP3 primary antibodies
  • Description

    Mouse monoclonal [MM0594-6E12] to ULBP3
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length Human ULBP3 protein

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and filtered through a 0.2 µm filter.
  • Clonality

    Monoclonal
  • Clone number

    MM0594-6E12
  • Isotype

    IgG2
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab89931 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 28 kDa.
IHC-P Use at an assay dependent concentration.

Target

  • Relevance

    ULBP3 is a human ligand for the NKG2D receptor, together with at least ULBP1 and ULBP2. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. ULBP3 has a lower affinity for NKG2D compared to ULBP1 and ULBP2 and induces weaker signaling responses than does ULBP2 or ULBP1.
  • Cellular localization

    Cell Membrane
  • Database links

  • Alternative names

    • ALCAN- gamma antibody
    • N2DL-3 antibody
    • N2DL3 antibody
    • NKG2D ligand 3 antibody
    • NKG2D ligand 3 precursor antibody
    • NKG2DL3 antibody
    • OTTHUMP00000017411 antibody
    • RAET1N antibody
    • Retinoic acid early transcript 1N antibody
    • UL16 binding protein 3 antibody
    • UL16-binding protein 3 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placental tissue section (4µm) labelling ULBP3 with ab89931. Tissue was treated with 15µg/ml Protenase for antigen retrieval and fixed with 4% PFA. A HRP-conjugated anti-mouse IgG was used as the secondary antibody (30 minutes at room temperature) followed by DAB color development.

References

This product has been referenced in:

  • Ma Y  et al. circRNA of AR-suppressed PABPC1 91 bp enhances the cytotoxicity of natural killer cells against hepatocellular carcinoma via upregulating UL16 binding protein 1. Oncol Lett 17:388-397 (2019). Read more (PubMed: 30655779) »
  • Cho H  et al. MICA/B and ULBP1 NKG2D ligands are independent predictors of good prognosis in cervical cancer. BMC Cancer 14:957 (2014). IHC-P ; Human . Read more (PubMed: 25510288) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

We used the recombinant protein (immunogen) to test the WB and we have the band at 25-30 kDa. I believe you were looking for a WB to test for the endogenous protein but unfortunately there is no WB data using a cell or tissue lysate. Please let me know if you have any further questions.

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Question
Answer

Thank you for your enquiry. I have submitted a request for a western blot using ab89931. Unfortunately I was informed that this information could not be provided until Monday. I apologize for the delay and will forward you any data I receive as soon as possible. Thanks for your patience.

Read More

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