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    ulk1-antibody-ab167139.pdf

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Signal Transduction Protein Phosphorylation Ser / Thr Kinases Other Kinases
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Anti-ULK1 antibody (ab167139)

  • Datasheet
Reviews (1) Submit a question References (7)

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Western blot - Anti-ULK1 antibody (ab167139)

    Key features and details

    • Rabbit polyclonal to ULK1
    • Suitable for: WB
    • Reacts with: Mouse, Rat, Human
    • Isotype: IgG

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    Overview

    • Product name

      Anti-ULK1 antibody
      See all ULK1 primary antibodies
    • Description

      Rabbit polyclonal to ULK1
    • Host species

      Rabbit
    • Specificity

      Two alternatively spliced transcript variants encoding different isoforms have been identified. ab167139 is predicted to not cross-react with ULK2.
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Mouse, Rat, Human
    • Immunogen

      Synthetic peptide, corresponding to 16 internal sequence amino acids of Human ULK1 (NP_003556).

    • Positive control

      • Rat brain tissue lysate.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
    • Storage buffer

      pH: 7.2
      Preservative: 0.02% Sodium azide
      Constituent: 99% PBS
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Protein Phosphorylation
      • Ser / Thr Kinases
      • Other Kinases
      • Neuroscience
      • Neurology process
      • Neurogenesis
      • Cardiovascular
      • Heart
      • Autophagy
      • Other
      • Metabolism
      • Pathways and Processes
      • Mitochondrial Metabolism
      • Mitophagy fission and fusion

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human ULK1 protein (ab95322)
    • Related Products

      • Recombinant Human ULK1 protein (ab95322)

    Applications

    Our Abpromise guarantee covers the use of ab167139 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use a concentration of 1 µg/ml. Predicted molecular weight: 112 kDa.

    Target

    • Function

      Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy (PubMed:25040165).
    • Tissue specificity

      Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.
    • Sequence similarities

      Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Autophosphorylated. Phosphorylated under nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. Under nutrient sufficiency, phosphorylated by MTOR/mTOR, disrupting the interaction with AMPK and preventing activation of ULK1 (By similarity). In response to nutrient limitation, phosphorylated and activated by AMPK, leading to activate autophagy.
    • Cellular localization

      Cytoplasm, cytosol. Preautophagosomal structure. Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome.
    • Target information above from: UniProt accession O75385 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 8408 Human
      • Entrez Gene: 22241 Mouse
      • Entrez Gene: 360827 Rat
      • Omim: 603168 Human
      • SwissProt: O75385 Human
      • SwissProt: O70405 Mouse
      • Unigene: 47061 Human
      • Unigene: 271898 Mouse
      • Unigene: 24509 Rat
      see all
    • Alternative names

      • ATG 1 antibody
      • ATG1 antibody
      • ATG1 autophagy related 1 homolog antibody
      • ATG1A antibody
      • Autophagy related protein 1 homolog antibody
      • Autophagy-related protein 1 homolog antibody
      • FLJ38455 antibody
      • FLJ46475 antibody
      • hATG1 antibody
      • KIAA0722 antibody
      • Serine/threonine protein kinase ULK1 antibody
      • Serine/threonine protein kinase Unc51.1 antibody
      • Serine/threonine-protein kinase ULK1 antibody
      • ULK 1 antibody
      • ULK1 antibody
      • ULK1_HUMAN antibody
      • Unc 51 (C. elegans) like kinase 1 antibody
      • UNC 51 antibody
      • Unc 51 like kinase 1 antibody
      • Unc-51 like kinase 1 (C. elegans) antibody
      • Unc-51-like kinase 1 antibody
      • UNC51 antibody
      • UNC51, C. elegans, homolog of antibody
      • Unc51.1 antibody
      see all

    Images

    • Western blot - Anti-ULK1 antibody (ab167139)
      Western blot - Anti-ULK1 antibody (ab167139)
      All lanes : Anti-ULK1 antibody (ab167139) at 1 µg/ml

      Lane 1 : Rat brain tissue lysate
      Lane 2 : Rat brain tissue lysate with blocking peptide

      Lysates/proteins at 15 µg per lane.

      Predicted band size: 112 kDa

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (7)

    Publishing research using ab167139? Please let us know so that we can cite the reference in this datasheet.

    ab167139 has been referenced in 7 publications.

    • Wang J  et al. Fundc1-dependent mitophagy is obligatory to ischemic preconditioning-conferred renoprotection in ischemic AKI via suppression of Drp1-mediated mitochondrial fission. Redox Biol 30:101415 (2020). PubMed: 31901590
    • You M  et al. 10-Hydroxydecanoic acid inhibits LPS-induced inflammation by targeting p53 in microglial cells. Int Immunopharmacol 84:106501 (2020). PubMed: 32311670
    • Zhu K  et al. LncRNA Sox2OT-V7 promotes doxorubicin-induced autophagy and chemoresistance in osteosarcoma via tumor-suppressive miR-142/miR-22. Aging (Albany NY) 12:6644-6666 (2020). PubMed: 32302291
    • Zhou XT  et al. Inhibition of autophagy enhances adenosine-induced apoptosis in human hepatoblastoma HepG2 cells. Oncol Rep 41:829-838 (2019). PubMed: 30535464
    • Chen X  et al. Wuhu Decoction Regulates Dendritic Cell Autophagy in the Treatment of Respiratory Syncytial Virus (RSV)-Induced Mouse Asthma by AMPK/ULK1 Signaling Pathway. Med Sci Monit 25:5389-5400 (2019). PubMed: 31325378
    • Gao XL  et al. Upregulation of microRNA-335-5p reduces inflammatory responses by inhibiting FASN through the activation of AMPK/ULK1 signaling pathway in a septic mouse model. Cytokine 110:466-478 (2018). PubMed: 29866515
    • Ibañez-Cabellos JS  et al. Extracellular histones activate autophagy and apoptosis via mTOR signaling in human endothelial cells. Biochim Biophys Acta Mol Basis Dis N/A:N/A (2018). PubMed: 30006152

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-ULK1 antibody

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (liver, brain, muscle whole tissue lysate)
    Gel Running Conditions
    Reduced Denaturing (8)
    Loading amount
    20 µg
    Treatment
    starvation vs non-starvation in mice
    Specification
    liver, brain, muscle whole tissue lysate
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Dr. Melanie Thelen

    Verified customer

    Submitted May 28 2019

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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