Overview

  • Product name

  • Description

    Rabbit polyclonal to ULK1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human ULK1 aa 567-577 (internal sequence).
    Sequence:

    RPKLPKPPTDP


    Database link: O75385

  • Positive control

    • ICC/IF: SK-N-BE cells. WB: HeLa and human 293Trap cell lysate; Rat kidney lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab240916 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000.
ICC/IF 1/100.

Target

  • Function

    Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy (PubMed:25040165).
  • Tissue specificity

    Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Autophosphorylated. Phosphorylated under nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. Under nutrient sufficiency, phosphorylated by MTOR/mTOR, disrupting the interaction with AMPK and preventing activation of ULK1 (By similarity). In response to nutrient limitation, phosphorylated and activated by AMPK, leading to activate autophagy.
  • Cellular localization

    Cytoplasm, cytosol. Preautophagosomal structure. Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • ATG 1 antibody
    • ATG1 antibody
    • ATG1 autophagy related 1 homolog antibody
    • ATG1A antibody
    • Autophagy related protein 1 homolog antibody
    • Autophagy-related protein 1 homolog antibody
    • FLJ38455 antibody
    • FLJ46475 antibody
    • hATG1 antibody
    • KIAA0722 antibody
    • Serine/threonine protein kinase ULK1 antibody
    • Serine/threonine protein kinase Unc51.1 antibody
    • Serine/threonine-protein kinase ULK1 antibody
    • ULK 1 antibody
    • ULK1 antibody
    • ULK1_HUMAN antibody
    • Unc 51 (C. elegans) like kinase 1 antibody
    • UNC 51 antibody
    • Unc 51 like kinase 1 antibody
    • Unc-51 like kinase 1 (C. elegans) antibody
    • Unc-51-like kinase 1 antibody
    • UNC51 antibody
    • UNC51, C. elegans, homolog of antibody
    • Unc51.1 antibody
    see all

Images

  • 4% formaldehyde-fixed SK-N-BE (human neuroblastoma cell line) cells labeling ULK1 (green) using ab240916 at 1/100 dilution in ICC/IF, followed by a goat anti-rabbit ATTO 488 conjugated secondary antibody at 1/200 dilution. Both primary and secondary antibodies were incubated for 1 hour at RT. Counterstained with Phalloidin Texas Red F-Actin stain (red) at 1/1000 for 1 hour at RT. The nuclear counterstain in DAPI (blue) at 1/5000 for 5 minutes at RT.

    (A) DAPI (blue) nuclear stain

    (B) Phalloidin Texas Red F-Actin stain (red)

    (C) ab240916

    (D) Composite

  • All lanes : Anti-ULK1 antibody (ab240916) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 2 : Human 293Trap cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : HRP-linked goat anti-rabbit at 1/2000 dilution

    Developed using the ECL technique.

    Exposure time: 6 minutes


    Blocked with 5% skim milk in 1XTBST.

  • Anti-ULK1 antibody (ab240916) at 1/1000 dilution + Rat kidney lysate at 15 µg

    Secondary
    HRP-linked goat anti-rabbit IgG at 1/2000 dilution

    Developed using the ECL technique.

    Exposure time: 6 minutes

References

ab240916 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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