Overview

  • Product name

    Anti-ULK1 (phospho S450) antibody [EPR6152]
    See all ULK1 primary antibodies
  • Description

    Rabbit monoclonal [EPR6152] to ULK1 (phospho S450)
  • Host species

    Rabbit
  • Specificity

    ab155971 only detects ULK1 phosphorylated at Serine 450.
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic phosphopeptide, corresponding to residues surrounding Serine 450 of Human ULK1 (Uniprot: O75385).

  • Positive control

    • 293T cell lysate transfected with ULK1.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab155971 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 113 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy (PubMed:25040165).
    • Tissue specificity

      Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.
    • Sequence similarities

      Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Autophosphorylated. Phosphorylated under nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. Under nutrient sufficiency, phosphorylated by MTOR/mTOR, disrupting the interaction with AMPK and preventing activation of ULK1 (By similarity). In response to nutrient limitation, phosphorylated and activated by AMPK, leading to activate autophagy.
    • Cellular localization

      Cytoplasm, cytosol. Preautophagosomal structure. Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome.
    • Information by UniProt
    • Database links

    • Alternative names

      • ATG 1 antibody
      • ATG1 antibody
      • ATG1 autophagy related 1 homolog antibody
      • ATG1A antibody
      • Autophagy related protein 1 homolog antibody
      • Autophagy-related protein 1 homolog antibody
      • FLJ38455 antibody
      • FLJ46475 antibody
      • hATG1 antibody
      • KIAA0722 antibody
      • Serine/threonine protein kinase ULK1 antibody
      • Serine/threonine protein kinase Unc51.1 antibody
      • Serine/threonine-protein kinase ULK1 antibody
      • ULK 1 antibody
      • ULK1 antibody
      • ULK1_HUMAN antibody
      • Unc 51 (C. elegans) like kinase 1 antibody
      • UNC 51 antibody
      • Unc 51 like kinase 1 antibody
      • Unc-51 like kinase 1 (C. elegans) antibody
      • Unc-51-like kinase 1 antibody
      • UNC51 antibody
      • UNC51, C. elegans, homolog of antibody
      • Unc51.1 antibody
      see all

    Images

    • All lanes : Anti-ULK1 (phospho S450) antibody [EPR6152] (ab155971) at 1/1000 dilution

      Lane 1 : 293T cell lysate transfected with ULK1.
      Lane 2 : 293T cell lysate transfected with ULK1, treated with Lambda Phosphatase

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 113 kDa

    References

    ab155971 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab155971.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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