Product nameAnti-ULK1 (phospho S467) antibody [EPR6153(2)-118]
See all ULK1 primary antibodies
DescriptionRabbit monoclonal [EPR6153(2)-118] to ULK1 (phospho S467)
Tested applicationsSuitable for: WBmore details
Unsuitable for: ICC/IF,IHC-P or IP
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ULK1 aa 450-550 (internal sequence) (phospho S467) (Cysteine residue). The exact sequence is proprietary.
Database link: O75385
- Lysate from 293T cells transfected with ULK1.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab179458 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 113 kDa.|
FunctionSerine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy (PubMed:25040165).
Tissue specificityUbiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.
Contains 1 protein kinase domain.
modificationsAutophosphorylated. Phosphorylated under nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. Under nutrient sufficiency, phosphorylated by MTOR/mTOR, disrupting the interaction with AMPK and preventing activation of ULK1 (By similarity). In response to nutrient limitation, phosphorylated and activated by AMPK, leading to activate autophagy.
Cellular localizationCytoplasm, cytosol. Preautophagosomal structure. Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome.
- Information by UniProt
- ATG 1 antibody
- ATG1 antibody
- ATG1 autophagy related 1 homolog antibody
All lanes : Anti-ULK1 (phospho S467) antibody [EPR6153(2)-118] (ab179458) at 1/1000 dilution
Lane 1 : Lysate from 293T cells transfected with ULK1. Membrane was not treated with Lambda Phosphatase.
Lane 2 : Lysate from 293T cells transfected with ULK1. Membrane was treated with Lambda Phosphatase.
Lysates/proteins at 10 µg per lane.
All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 113 kDa
Bottom panel: lysates were labeled with ab128859, a non-phospho specific anti-ULK1 antibody.
ab179458 has not yet been referenced specifically in any publications.