Overview

  • Product name

    Anti-ULK1 (phospho S556) antibody
    See all ULK1 primary antibodies
  • Description

    Rabbit polyclonal to ULK1 (phospho S556)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ULK1 aa 530-580 (phospho S556) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Sequence:

    GGRSPRPGSSAPEHSPRTSGLGCRLHSAPNLSDLHVVRPKLPKPPTDPLG A


    Database link: O75385

  • Positive control

    • Rat brain tissue; Mouse brain and heart tissue lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab203207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Use at 1/50 - 1/200 with fluorescent detection methods.

WB 1/100 - 1/1000. Predicted molecular weight: 112 kDa.

Target

  • Function

    Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy (PubMed:25040165).
  • Tissue specificity

    Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Autophosphorylated. Phosphorylated under nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. Under nutrient sufficiency, phosphorylated by MTOR/mTOR, disrupting the interaction with AMPK and preventing activation of ULK1 (By similarity). In response to nutrient limitation, phosphorylated and activated by AMPK, leading to activate autophagy.
  • Cellular localization

    Cytoplasm, cytosol. Preautophagosomal structure. Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • ATG 1 antibody
    • ATG1 antibody
    • ATG1 autophagy related 1 homolog antibody
    • ATG1A antibody
    • Autophagy related protein 1 homolog antibody
    • Autophagy-related protein 1 homolog antibody
    • FLJ38455 antibody
    • FLJ46475 antibody
    • hATG1 antibody
    • KIAA0722 antibody
    • Serine/threonine protein kinase ULK1 antibody
    • Serine/threonine protein kinase Unc51.1 antibody
    • Serine/threonine-protein kinase ULK1 antibody
    • ULK 1 antibody
    • ULK1 antibody
    • ULK1_HUMAN antibody
    • Unc 51 (C. elegans) like kinase 1 antibody
    • UNC 51 antibody
    • Unc 51 like kinase 1 antibody
    • Unc-51 like kinase 1 (C. elegans) antibody
    • Unc-51-like kinase 1 antibody
    • UNC51 antibody
    • UNC51, C. elegans, homolog of antibody
    • Unc51.1 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed and paraffin embedded Rat brain tissue labeling ULK1 (phospho S556) using ab203207 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

  • All lanes : Anti-ULK1 (phospho S556) antibody (ab203207) at 1/200 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse heart tissue lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/3000 dilution

    Predicted band size: 112 kDa



    12% Gel.

References

This product has been referenced in:

  • Zhu X  et al. Silencing of SNHG6 induced cell autophagy by targeting miR-26a-5p/ULK1 signaling pathway in human osteosarcoma. Cancer Cell Int 19:82 (2019). Read more (PubMed: 30988663) »
  • Zhu H  et al. ALKBH5 inhibited autophagy of epithelial ovarian cancer through miR-7 and BCL-2. J Exp Clin Cancer Res 38:163 (2019). Read more (PubMed: 30987661) »
See all 4 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (Prostate cancer cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Prostate cancer cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Dr. Dimitra Kalamida

Verified customer

Submitted Jul 11 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (DU145 Prostate cancer cell line)
Permeabilization
Yes - 0.1% Triton X-100
Specification
DU145 Prostate cancer cell line
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: Rt°C
Fixative
Paraformaldehyde

Dr. Dimitra Kalamida

Verified customer

Submitted Sep 07 2018

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