Overview

  • Product name

    Anti-ULK1 (phospho S556) antibody [EPR6156]
    See all ULK1 primary antibodies
  • Description

    Rabbit monoclonal [EPR6156] to ULK1 (phospho S556)
  • Host species

    Rabbit
  • Specificity

    ab133747 only detects ULK1 phosphorylated at Serine 556.
  • Tested applications

    Suitable for: WB, Dot blotmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Phospho-synthetic peptide from a region surrounding Serine 556 of Human ULK1

  • Positive control

    • ULK1 transfected 293T cell lysate
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 120 kDa (predicted molecular weight: 112 kDa).
Dot blot Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function

      Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy (PubMed:25040165).
    • Tissue specificity

      Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.
    • Sequence similarities

      Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Autophosphorylated. Phosphorylated under nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. Under nutrient sufficiency, phosphorylated by MTOR/mTOR, disrupting the interaction with AMPK and preventing activation of ULK1 (By similarity). In response to nutrient limitation, phosphorylated and activated by AMPK, leading to activate autophagy.
    • Cellular localization

      Cytoplasm, cytosol. Preautophagosomal structure. Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome.
    • Information by UniProt
    • Database links

    • Alternative names

      • ATG 1 antibody
      • ATG1 antibody
      • ATG1 autophagy related 1 homolog antibody
      • ATG1A antibody
      • Autophagy related protein 1 homolog antibody
      • Autophagy-related protein 1 homolog antibody
      • FLJ38455 antibody
      • FLJ46475 antibody
      • hATG1 antibody
      • KIAA0722 antibody
      • Serine/threonine protein kinase ULK1 antibody
      • Serine/threonine protein kinase Unc51.1 antibody
      • Serine/threonine-protein kinase ULK1 antibody
      • ULK 1 antibody
      • ULK1 antibody
      • ULK1_HUMAN antibody
      • Unc 51 (C. elegans) like kinase 1 antibody
      • UNC 51 antibody
      • Unc 51 like kinase 1 antibody
      • Unc-51 like kinase 1 (C. elegans) antibody
      • Unc-51-like kinase 1 antibody
      • UNC51 antibody
      • UNC51, C. elegans, homolog of antibody
      • Unc51.1 antibody
      see all

    Images

    • All lanes : Anti-ULK1 (phospho S556) antibody [EPR6156] (ab133747) at 1/10000 dilution

      Lane 1 : ULK1 transfected 293T cell lysate untreated
      Lane 2 : ULK1 transfected 293T cell lysate treated with Lambda Phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 112 kDa
      Observed band size: 120 kDa
      why is the actual band size different from the predicted?



      The bottom panel shows total levels of ULK1 in both samples.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • All lanes: Anti-ULK1 (phospho S556) antibody [EPR6156] (ab133747) at 1/1000 dilution

      Lane 1 : ULK1 (pS556) phospho peptide

      Lane 2 : ULK1 non-phospho peptide

       

      Secondary antibody: Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 112 kDa

      Exposure time: 3 minutes

      Blocking and diluting buffer used was 5% NFDM/TBST.

       

    • All lanes : Anti-ULK1 (phospho S556) antibody [EPR6156] (ab133747) at 1/5000 dilution

      Lane 1 : 293 (Human embryonic kidney epithelial cell) whole cell lysates
      Lane 2 : 293 (Human embryonic kidney epithelial cell) treated with epidermal growth factor.

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 112 kDa
      Observed band size: 120 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer used was 5% NFDM/TBST.

    References

    This product has been referenced in:

    • Zhang F  et al. High-mobility group protein N2 induces autophagy by activating AMPK/ULK1 pathway and thereby boosts UPEC proliferation within bladder epithelial cells. Biochem Biophys Res Commun 513:193-200 (2019). Read more (PubMed: 30952427) »
    • Zhao X  et al. Ozone induces autophagy in rat chondrocytes stimulated with IL-1ß through the AMPK/mTOR signaling pathway. J Pain Res 11:3003-3017 (2018). Read more (PubMed: 30568481) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133747.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up