Product nameAnti-ULK1 (phospho S638) antibody
See all ULK1 primary antibodies
DescriptionRabbit polyclonal to ULK1 (phospho S638)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human ULK1 (internal sequence) (phospho S638). The exact sequence is proprietary.
Database link: O75385
- WB: MCF7 whole cell extract.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 1% BSA, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229755 in the following tested applications.
|WB||1/500 - 1/3000. Predicted molecular weight: 113 kDa.|
FunctionSerine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy (PubMed:25040165).
Tissue specificityUbiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.
Contains 1 protein kinase domain.
modificationsAutophosphorylated. Phosphorylated under nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. Under nutrient sufficiency, phosphorylated by MTOR/mTOR, disrupting the interaction with AMPK and preventing activation of ULK1 (By similarity). In response to nutrient limitation, phosphorylated and activated by AMPK, leading to activate autophagy.
Cellular localizationCytoplasm, cytosol. Preautophagosomal structure. Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome.
- Information by UniProt
- ATG 1 antibody
- ATG1 antibody
- ATG1 autophagy related 1 homolog antibody
All lanes : Anti-ULK1 (phospho S638) antibody (ab229755) at 1/1000 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell extract
Lane 2 : MCF7 whole cell extract treated with CIP
Lysates/proteins at 50 µg per lane.
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 113 kDa
7.5% SDS-PAGE gel.
ab229755 has not yet been referenced specifically in any publications.