Overview

  • Product name
    Anti-ULK1 (phospho S758) antibody
    See all ULK1 primary antibodies
  • Description
    Rabbit polyclonal to ULK1 (phospho S758)
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Chimpanzee, Macaque monkey, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human ULK1 aa 750-850 (phospho S758) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: O75385

  • Positive control
    • This antibody gave a positive signal in A431 and A431 EGF treated (500mg) whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab156920 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa).
ELISA Use a concentration of 1 µg/ml.

Target

  • Function
    Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy (PubMed:25040165).
  • Tissue specificity
    Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated. Phosphorylated under nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. Under nutrient sufficiency, phosphorylated by MTOR/mTOR, disrupting the interaction with AMPK and preventing activation of ULK1 (By similarity). In response to nutrient limitation, phosphorylated and activated by AMPK, leading to activate autophagy.
  • Cellular localization
    Cytoplasm, cytosol. Preautophagosomal structure. Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATG 1 antibody
    • ATG1 antibody
    • ATG1 autophagy related 1 homolog antibody
    • ATG1A antibody
    • Autophagy related protein 1 homolog antibody
    • Autophagy-related protein 1 homolog antibody
    • FLJ38455 antibody
    • FLJ46475 antibody
    • hATG1 antibody
    • KIAA0722 antibody
    • Serine/threonine protein kinase ULK1 antibody
    • Serine/threonine protein kinase Unc51.1 antibody
    • Serine/threonine-protein kinase ULK1 antibody
    • ULK 1 antibody
    • ULK1 antibody
    • ULK1_HUMAN antibody
    • Unc 51 (C. elegans) like kinase 1 antibody
    • UNC 51 antibody
    • Unc 51 like kinase 1 antibody
    • Unc-51 like kinase 1 (C. elegans) antibody
    • Unc-51-like kinase 1 antibody
    • UNC51 antibody
    • UNC51, C. elegans, homolog of antibody
    • Unc51.1 antibody
    see all

Images

  • All lanes : Anti-ULK1 (phospho S758) antibody (ab156920) at 1 µg/ml

    Lane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : EGF-Stimulated A431 Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate with Immunising peptide at 1 µg/ml
    Lane 4 : EGF-Stimulated A431 Whole Cell Lysate with Immunising peptide at 1 µg/ml
    Lane 5 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate with Control peptide at 1 µg/ml
    Lane 6 : EGF-Stimulated A431 Whole Cell Lysate with Control peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 112 kDa
    Observed band size: 112 kDa
    Additional bands at: 130 kDa (possible non-specific binding)


    Exposure time: 8 minutes


    This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab156920 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

  • ab156920 was tested using an Indirect ELISA approach. The wells were coated with peptide (1µg/ml at 100µl/well) overnight at 4°C, followed by a 5% BSA blocking step for 1 hour at room temperature. The primary Ab was then added at a dilution range of 1- 0.00025µg/ml (100µl/well) for 1hr at room temperature. A HRP-conjugated anti-rabbit IgG (heavy and light chain) was used as a secondary antibody at 1:20,000 dilution for 1hr at room temperature.

References

ab156920 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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