Overview

  • Product name

    Anti-ULK1 (phospho S758) antibody
    See all ULK1 primary antibodies
  • Description

    Rabbit polyclonal to ULK1 (phospho S758)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human ULK1 (internal sequence) (phospho S758). The exact sequence is proprietary. Carrier-protein conjugated.
    Database link: O75385

  • Positive control

    • WB: MCF7 whole cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab229581 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 113 kDa.

Target

  • Function

    Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy (PubMed:25040165).
  • Tissue specificity

    Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Autophosphorylated. Phosphorylated under nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. Under nutrient sufficiency, phosphorylated by MTOR/mTOR, disrupting the interaction with AMPK and preventing activation of ULK1 (By similarity). In response to nutrient limitation, phosphorylated and activated by AMPK, leading to activate autophagy.
  • Cellular localization

    Cytoplasm, cytosol. Preautophagosomal structure. Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • ATG 1 antibody
    • ATG1 antibody
    • ATG1 autophagy related 1 homolog antibody
    • ATG1A antibody
    • Autophagy related protein 1 homolog antibody
    • Autophagy-related protein 1 homolog antibody
    • FLJ38455 antibody
    • FLJ46475 antibody
    • hATG1 antibody
    • KIAA0722 antibody
    • Serine/threonine protein kinase ULK1 antibody
    • Serine/threonine protein kinase Unc51.1 antibody
    • Serine/threonine-protein kinase ULK1 antibody
    • ULK 1 antibody
    • ULK1 antibody
    • ULK1_HUMAN antibody
    • Unc 51 (C. elegans) like kinase 1 antibody
    • UNC 51 antibody
    • Unc 51 like kinase 1 antibody
    • Unc-51 like kinase 1 (C. elegans) antibody
    • Unc-51-like kinase 1 antibody
    • UNC51 antibody
    • UNC51, C. elegans, homolog of antibody
    • Unc51.1 antibody
    see all

Images

  • All lanes : Anti-ULK1 (phospho S758) antibody (ab229581) at 1/500 dilution

    Lane 1 : Untreated MCF7 (human breast adenocarcinoma cell line) whole cell extract
    Lane 2 : MCF7 (human breast adenocarcinoma cell line) treated with 200 nM Rapamycin for 0.5 hours and starved for 16 hours, whole cell extract

    Lysates/proteins at 60 µg per lane.

    Predicted band size: 113 kDa



    7.5% SDS-PAGE gel.

References

ab229581 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - whole (brain, liver, muscle whole tissue lysate)
Gel Running Conditions
Reduced Denaturing (8 %)
Loading amount
20 µg
Treatment
mouse starvation vs non-starvation
Specification
brain, liver, muscle whole tissue lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Dr. Melanie Thelen

Verified customer

Submitted May 24 2019

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