Overview

  • Product name

    Anti-ULK2 antibody
  • Description

    Rabbit polyclonal to ULK2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 693 and 938 of Human ULK2 (NP_055498).

  • Positive control

    • 293T, H1299, HeLa, Molt-4, Raji cell lysates. HepG2 whole cell lysates. HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 113 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Autophosphorylated.
  • Information by UniProt
  • Database links

  • Alternative names

    • ATG1B antibody
    • KIAA0623 antibody
    • Serine/threonine protein kinase ULK2 antibody
    • Serine/threonine-protein kinase ULK2 antibody
    • ULK2 antibody
    • ULK2_HUMAN antibody
    • Unc 51 (C. elegans) like kinase 2 antibody
    • Unc 51 like autophagy activating kinase 2 antibody
    • Unc 51 like kinase 2 antibody
    • Unc-51-like kinase 2 antibody
    • Unc51.2 antibody
    see all

Images

  • Anti-ULK2 antibody (ab97695) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 113 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab97695 at 1/200 dilution. Lower image is merged with DNA probe.

References

This product has been referenced in:

  • Blessing AM  et al. Transcriptional regulation of core autophagy and lysosomal genes by the androgen receptor promotes prostate cancer progression. Autophagy 13:506-521 (2017). Read more (PubMed: 27977328) »
  • Puto LA  et al. Transcriptional Repressor DAXX Promotes Prostate Cancer Tumorigenicity via Suppression of Autophagy. J Biol Chem 290:15406-20 (2015). WB ; Human . Read more (PubMed: 25903140) »
See all 3 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - other (cortex)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
cortex
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Sep 11 2017

Answer

Thank you for your reply, and for your patience in resolving this issue. I have asked that these three replacement vials be added to your next shipment. Please let me know if you have any questions or if there is anything else that we can do for you.

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Question
Answer

I have processed your request to receive ab56736 as a replacement for ab97695. You r new order number is ******.

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Answer

I am sorry that the protocol tips did not prove to be successful and I would be happy to send you ab56736 as a replacement. I would just like to confirm the original order information. Can you confirm that it was ordered by yourself on*******. I just like to confirm that I have the original details to make sure there are no mistakes.

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Question
Answer

Thank you for your reply. I would be happy to send replacements. Does the customer wish to try fresh vials of the antibodies they have already tried, or would they prefer different antibodies to the targets NPTX2, NUCKS1, and ULK2? I look forward to hearing from you, and I will send these replacements out promptly.

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Answer

Thank you for contacting us and for confirming the details of your experiment. I am sorry that this antibody did not perform as stated on the datasheet and your protocol seems fine. This amount will be refunded to the credit card used to make this purchase. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. The credit note ID is for your reference only and does not automatically guarantee the credit. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.  

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Answer

Thank you for your reply. I would be happy to offer the customer a free of charge replacement or credit. Please let me know how the customer would like to proceed and if they would like a replacement, which product they would like to receive. I would be happy to help you further once I have this additional information.

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Answer

Thanks for your reply and for sending the additional information. I do have a couple of additional suggestions, however I am not sure if the customer has tried these steps already. For the antibodies ab97695, ab69858, and ab7770, we would recommend diluting around 1:2000-1:3000. We would also recommend blocking the membrane in 5% BSA overnight to reduce non-specific binding. If the customer has already tried similar optimization steps, I would be happy to send replacement antibodies or alternatively issue a credit note. We would also be very grateful if the customer could provide an image of the results so that we may investigate the problem with these antibodies. I look forward to hearing from you. Please let me know if you have any questions.

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