Key features and details
- Rabbit polyclonal to ULK3
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ULK3 antibody
See all ULK3 primary antibodies
DescriptionRabbit polyclonal to ULK3
Specificityab219264 is Human specific. Multiple isoforms of ULK3 are known to exist.
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human ULK3 (internal sequence). The exact sequence is proprietary. (19 amino acid peptide).
Database link: Q6PHR2
- IHC-P; Human liver tissue. IF; Human brain tissue. WB; Human brain tissue lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab219264 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 20 µg/ml.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 53 kDa.|
FunctionSerine/threonine protein kinase which enhances GLI1 and GLI2 transcriptional activity and consequently positively regulates GLI-dependent SHH signaling. May exert this function by promoting GLI1 nuclear localization. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently.
Tissue specificityWidely expressed. Highest levels observed in fetal brain. In adult tissues, high levels in brain, liver and kidney, moderate levels in testis and adrenal gland and low levels in heart, lung, stomach, thymus, prostate and placenta. In the brain, highest expression in the hippocampus, high levels also detected in the cerebellum, olfactory bulb and optice nerve. In the central nervous system, lowest levels in the spinal cord.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.
Contains 2 MIT domains.
Contains 1 protein kinase domain.
modificationsAutophosphorylated in vitro.
- Information by UniProt
FormThere are 3 isoforms produced by alternative splicing.
- DKFZP434C131 antibody
- FLJ90566 antibody
- Serine/threonine-protein kinase ULK3 antibody
Lane 1 : Anti-ULK3 antibody (ab219264) at 0.5 µg/ml
Lane 2 : Anti-ULK3 antibody (ab219264) at 1 µg/ml
All lanes : Human brain tissue lysate
Predicted band size: 53 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue labeling ULK3 with ab219264 at 10 µg/ml.
Immunofluorescent analysis of Human brain tissue labeling ULK3 with ab219264 at 20 µg/ml.
ab219264 has not yet been referenced specifically in any publications.