Overview

  • Product name

  • Description

    Rabbit polyclonal to ULK3
  • Host species

    Rabbit
  • Specificity

    ab219264 is Human specific. Multiple isoforms of ULK3 are known to exist.
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human ULK3 (internal sequence). The exact sequence is proprietary. (19 amino acid peptide).
    Database link: Q6PHR2

  • Positive control

    • IHC-P; Human liver tissue. IF; Human brain tissue. WB; Human brain tissue lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab219264 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 53 kDa.

Target

  • Function

    Serine/threonine protein kinase which enhances GLI1 and GLI2 transcriptional activity and consequently positively regulates GLI-dependent SHH signaling. May exert this function by promoting GLI1 nuclear localization. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently.
  • Tissue specificity

    Widely expressed. Highest levels observed in fetal brain. In adult tissues, high levels in brain, liver and kidney, moderate levels in testis and adrenal gland and low levels in heart, lung, stomach, thymus, prostate and placenta. In the brain, highest expression in the hippocampus, high levels also detected in the cerebellum, olfactory bulb and optice nerve. In the central nervous system, lowest levels in the spinal cord.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.
    Contains 2 MIT domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Autophosphorylated in vitro.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Form

    There are 3 isoforms produced by alternative splicing.
  • Alternative names

    • DKFZP434C131 antibody
    • FLJ90566 antibody
    • Serine/threonine-protein kinase ULK3 antibody
    • Ulk3 antibody
    • ULK3_HUMAN antibody
    • unc 51 like kinase 3 (C. elegans) antibody
    • Unc-51-like kinase 3 antibody
    see all

Images

  • Lane 1 : Anti-ULK3 antibody (ab219264) at 0.5 µg/ml
    Lane 2 : Anti-ULK3 antibody (ab219264) at 1 µg/ml

    All lanes : Human brain tissue lysate

    Predicted band size: 53 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue labeling ULK3 with ab219264 at 10 µg/ml.

  • Immunofluorescent analysis of Human brain tissue labeling ULK3 with ab219264 at 20 µg/ml.

References

ab219264 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab219264.
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