Product nameAnti-ULK3 antibody [EPR4888]
See all ULK3 primary antibodies
DescriptionRabbit monoclonal [EPR4888] to ULK3
Tested applicationsSuitable for: WB, ICC, Flow Cyt, IHC-Pmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Synthetic peptide within Human ULK3 aa 400-500 (C terminal). The exact sequence is proprietary.
- IHC-P: Human bladder carcinoma tissue WB: Human heart, testis, fetal kidney, LnCaP and 293T cell lysates
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124947 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 53 kDa.|
|ICC||1/25 - 1/50.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/50 - 1/100.|
FunctionSerine/threonine protein kinase which enhances GLI1 and GLI2 transcriptional activity and consequently positively regulates GLI-dependent SHH signaling. May exert this function by promoting GLI1 nuclear localization. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently.
Tissue specificityWidely expressed. Highest levels observed in fetal brain. In adult tissues, high levels in brain, liver and kidney, moderate levels in testis and adrenal gland and low levels in heart, lung, stomach, thymus, prostate and placenta. In the brain, highest expression in the hippocampus, high levels also detected in the cerebellum, olfactory bulb and optice nerve. In the central nervous system, lowest levels in the spinal cord.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.
Contains 2 MIT domains.
Contains 1 protein kinase domain.
modificationsAutophosphorylated in vitro.
- Information by UniProt
FormThere are 3 isoforms produced by alternative splicing.
- DKFZP434C131 antibody
- FLJ90566 antibody
- Serine/threonine-protein kinase ULK3 antibody
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: ULK3 knockout HAP1 whole cell lysate (20 µg)
Lane 3: Hek293 whole cell lysate (20 µg)
Lanes 1 - 3: Merged signal (red and green). Green - ab124947 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab124947 was shown to specifically react with ULK3 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when ULK3 knockout samples were examined. Wild-type and ULK3 knockout samples were subjected to SDS-PAGE. Ab124947 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
All lanes : Anti-ULK3 antibody [EPR4888] (ab124947) at 1/1000 dilution
Lane 1 : Human heart lysate
Lane 2 : Human testis lysate
Lane 3 : Human fetal kidney lysate
Lane 4 : LnCaP cell lysate
Lane 5 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 53 kDa
ab124947, at a 1/50 dilution, staining ULK3 in paraffin embedded Human bladder carcinoma tissue by Immunohistochemistry.