Overview

  • Product name
  • Description
    Goat polyclonal to UMOD
  • Host species
    Goat
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human UMOD aa 525-536 (internal sequence).
    Sequence:

    DRCPHTRDSTIQ


    Database link: NP_003352.2

  • Positive control
    • HeLa cell lysate.
  • General notes

    Previously labelled as Uromucoid. 

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab156827 was purified from serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab156827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 70 kDa).

Target

  • Function
    Not known. May play a role in regulating the circulating activity of cytokines as it binds to IL-1, IL-2 and TNF with high affinity.
  • Tissue specificity
    Synthesized by kidney. Most abundant protein in normal human urine.
  • Involvement in disease
    Defects in UMOD are the cause of familial juvenile hyperuricemic nephropathy type 1 (HNFJ1) [MIM:162000]. HNFJ1 is a renal disease characterized by juvenil onset of hyperuricemia, polyuria, progressive renal failure, and gout. The disease is associated with interstitial pathological changes resulting in fibrosis.
    Defects in UMOD are the cause of medullary cystic kidney disease type 2 (MCKD2) [MIM:603860]. MCKD2 is a form of tubulointerstitial nephropathy characterized by formation of renal cysts at the corticomedullary junction. It is characterized by adult onset of impaired renal function and salt wasting resulting in end-stage renal failure by the sixth decade.
    Defects in UMOD are the cause of glomerulocystic kidney disease with hyperuricemia and isosthenuria (GCKDHI) [MIM:609886]. GCKDHI is a renal disorder characterized by a cystic dilation of Bowman space, a collapse of glomerular tuft, and hyperuricemia due to low fractional excretion of uric acid and severe impairment of urine concentrating ability.
  • Sequence similarities
    Contains 3 EGF-like domains.
    Contains 1 ZP domain.
  • Cellular localization
    Cell membrane. Secreted. Secreted after cleavage in the urine.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADMCKD2 antibody
    • FJHN antibody
    • HNFJ antibody
    • HNFJ1 antibody
    • MCKD2 antibody
    • medullary cystic kidney disease 2 (autosomal dominant) antibody
    • Tamm Horsfall glycoprotein antibody
    • Tamm Horsfall urinary glycoprotein antibody
    • Tamm-Horsfall urinary glycoprotein antibody
    • THGP antibody
    • THP antibody
    • Umod antibody
    • Urehd1 antibody
    • urehr4 antibody
    • UROM_HUMAN antibody
    • uromodulin (uromucoid, Tamm-Horsfall glycoprotein) antibody
    • Uromodulin antibody
    • Uromodulin, secreted form antibody
    • Uromucoid antibody
    see all

Images

  • Anti-UMOD antibody (ab156827) at 0.5 µg/ml + HeLa cell lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 70 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

References

ab156827 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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