Overview

  • Product name

  • Description

    Rabbit polyclonal to UMOD
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Mouse UMOD aa 375-420 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    ERDWMS IVTPARNGPC GTVLRRNETH ATYSNTLYLA NAIIIRDIII


    Database link: Q91X17

  • Positive control

    • Rat and mouse kidney lysates; human kidney carcinoma lysate; mouse kidney tissue.
  • General notes

    Previously labelled as Uromucoid. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 1% BSA, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab217354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/1000. Predicted molecular weight: 71 kDa.
IHC-P 1/100 - 1/500.

Target

  • Function

    Not known. May play a role in regulating the circulating activity of cytokines as it binds to IL-1, IL-2 and TNF with high affinity.
  • Tissue specificity

    Synthesized by kidney. Most abundant protein in normal human urine.
  • Involvement in disease

    Defects in UMOD are the cause of familial juvenile hyperuricemic nephropathy type 1 (HNFJ1) [MIM:162000]. HNFJ1 is a renal disease characterized by juvenil onset of hyperuricemia, polyuria, progressive renal failure, and gout. The disease is associated with interstitial pathological changes resulting in fibrosis.
    Defects in UMOD are the cause of medullary cystic kidney disease type 2 (MCKD2) [MIM:603860]. MCKD2 is a form of tubulointerstitial nephropathy characterized by formation of renal cysts at the corticomedullary junction. It is characterized by adult onset of impaired renal function and salt wasting resulting in end-stage renal failure by the sixth decade.
    Defects in UMOD are the cause of glomerulocystic kidney disease with hyperuricemia and isosthenuria (GCKDHI) [MIM:609886]. GCKDHI is a renal disorder characterized by a cystic dilation of Bowman space, a collapse of glomerular tuft, and hyperuricemia due to low fractional excretion of uric acid and severe impairment of urine concentrating ability.
  • Sequence similarities

    Contains 3 EGF-like domains.
    Contains 1 ZP domain.
  • Cellular localization

    Cell membrane. Secreted. Secreted after cleavage in the urine.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADMCKD2 antibody
    • FJHN antibody
    • HNFJ antibody
    • HNFJ1 antibody
    • MCKD2 antibody
    • medullary cystic kidney disease 2 (autosomal dominant) antibody
    • Tamm Horsfall glycoprotein antibody
    • Tamm Horsfall urinary glycoprotein antibody
    • Tamm-Horsfall urinary glycoprotein antibody
    • THGP antibody
    • THP antibody
    • Umod antibody
    • Urehd1 antibody
    • urehr4 antibody
    • UROM_HUMAN antibody
    • uromodulin (uromucoid, Tamm-Horsfall glycoprotein) antibody
    • Uromodulin antibody
    • Uromodulin, secreted form antibody
    • Uromucoid antibody
    see all

Images

  • Anti-UMOD antibody (ab217354) at 1/300 dilution + Mouse kidney lysate

    Secondary
    Goat Anti-Rabbit IgG Antibody (H+L), HRP at 1/5000 dilution

    Predicted band size: 71 kDa

  • All lanes : Anti-UMOD antibody (ab217354) at 1/200 dilution

    Lane 1 : rat kidney lysate
    Lane 2 : human kidney carcinoma lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HRP at 1/3000 dilution

    Predicted band size: 71 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse kidney tissue labeling UMOD with ab217354 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.

References

This product has been referenced in:

  • Han WQ  et al. Membrane rafts-redox signalling pathway contributes to renal fibrosis via modulation of the renal tubular epithelial-mesenchymal transition. J Physiol 596:3603-3616 (2018). Read more (PubMed: 29863758) »
See 1 Publication for this product

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