Anti-Nucleoside-diphosphate kinase antibody (ab228609)
Key features and details
- Rabbit polyclonal to Nucleoside-diphosphate kinase
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Nucleoside-diphosphate kinase antibody
See all Nucleoside-diphosphate kinase primary antibodies -
Description
Rabbit polyclonal to Nucleoside-diphosphate kinase -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Rhesus monkey -
Immunogen
Recombinant fragment within Human Nucleoside-diphosphate kinase (internal sequence). The exact sequence is proprietary.
Database link: P30085 -
Positive control
- WB: Raji whole cell lysate; mouse brain tissue whole cell lysate. ICC/IF: HCT 116 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228609 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 22 kDa.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 22 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Catalyzes specific phosphoryl transfer from ATP to UMP and CMP. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the adenylate kinase family. -
Cellular localization
Nucleus. Cytoplasm. Mainly nuclear. - Information by UniProt
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Database links
- Entrez Gene: 429100 Chicken
- Entrez Gene: 509965 Cow
- Entrez Gene: 51727 Human
- Entrez Gene: 66588 Mouse
- Entrez Gene: 298410 Rat
- Entrez Gene: 710196 Rhesus monkey
- Omim: 191710 Human
- SwissProt: Q2KIW9 Cow
see all -
Alternative names
- CMK antibody
- CMPK 1 antibody
- CMPK antibody
see all
Images
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HCT 116 (human colorectal carcinoma cell line) cells stained for Nucleoside-diphosphate kinase (green) using ab228609 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Blue: Hoechst 33342 staining.
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Anti-Nucleoside-diphosphate kinase antibody (ab228609) at 1/500 dilution + Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 22 kDa12% SDS-PAGE
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Anti-Nucleoside-diphosphate kinase antibody (ab228609) at 1/1000 dilution + Mouse brain tissue whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 22 kDa12% SDS-PAGE
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab228609 has not yet been referenced specifically in any publications.