Anti-Pol V antibody (ab91343)
Key features and details
- Rabbit polyclonal to Pol V
- Suitable for: WB
- Reacts with: Escherichia coli
- Isotype: IgG
Overview
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Product name
Anti-Pol V antibody -
Description
Rabbit polyclonal to Pol V -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Escherichia coli -
Immunogen
Fusion protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Protein extract from E. coli
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Preservative: 0.05% Sodium azide
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab91343 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/3000. Predicted molecular weight: 14 kDa.
Predicted molecular weight: 14 kDa (processed form. Precursor form is 17 kDa). |
Notes |
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WB
1/3000. Predicted molecular weight: 14 kDa. Predicted molecular weight: 14 kDa (processed form. Precursor form is 17 kDa). |
Target
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Relevance
UmuD is a DNA polymerase V subunit capable of translesion synthesis and inducible mutagenesis, by radiation or chemical agents. Transcription of umuD is repressed by a repressor, LexA protein in uninduced cells. The processed UmuD protein is the active form for mutagenesis and the UmuD-UmuC complex functions as a error-prone translesion DNA polymerase. The molecular weight of the intact UmuD is 17kD and the proteolytically processed active form is 14KD. -
Database links
- Entrez Gene: 945746 Escherichia coli
- SwissProt: P0AG11 Escherichia coli
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Alternative names
- DNA polymerase V subunit D antibody
- ECK1171 antibody
- JW1172 antibody
- Protein umuD antibody
Images
Datasheets and documents
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Datasheet download
References (2)
ab91343 has been referenced in 2 publications.
- Naiman K et al. Chronology in lesion tolerance gives priority to genetic variability. Proc Natl Acad Sci U S A 111:5526-31 (2014). PubMed: 24706928
- Cafarelli TM et al. A single residue unique to DinB-like proteins limits formation of the polymerase IV multiprotein complex in Escherichia coli. J Bacteriol 195:1179-93 (2013). Escherichia coli . PubMed: 23292773