Overview

  • Product name

    Anti-UNC119-binding protein C5orf30 antibody
  • Description

    Rabbit polyclonal to UNC119-binding protein C5orf30
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Guinea pig, Cow, Pig, Chimpanzee, Non human primates, Gorilla, Orangutan
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1-77 of Human UNC119-binding protein C5orf30 (Q96GV9).

  • Positive control

    • Human thyroid gland tissue
  • General notes

    The antibody solution should be gently mixed before use.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab150906 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/250.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Function

    Probably plays a role in trafficking of proteins via its interaction with UNC119 and UNC119B cargo adapters: may help the release of UNC119 and UNC119B cargo or the recycling of UNC119 and UNC119B. May play a role in ciliary membrane localization via its interaction with UNC119B and protein transport into photoreceptor cells.
  • Sequence similarities

    Belongs to the UNC119-binding protein family.
  • Cellular localization

    Cytoplasm. Cell projection > cilium. Localizes to the transition zone and proximal cilium in addition to being found throughout the cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • C5orf30 antibody
    • CE030_HUMAN antibody
    • chromosome 5 open reading frame 30 antibody
    • D1Ertd622e antibody
    • FLJ25291 antibody
    • UNC119-binding protein C5orf30 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleoli and mitochondria. Recommended concentration of ab150906 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Lanes 2-3 : Anti-UNC119-binding protein C5orf30 antibody (ab150906)

    Lane 1 : Marker
    Lane 2 : HEK293T cell lysate - vector only transfected
    Lane 3 : HEK293T cell lysate - overexpressing UNC119-binding protein C5orf30 with a C-terminal myc-DDK tag (~3.1kDa)
  • Immunohistochemical analysis of paraffin embedded Human thyroid gland tissue labeling UNC119-binding protein C5orf30 with ab150906 antibody at 1/200.

References

ab150906 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab150906.
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