Key features and details
- Rabbit polyclonal to UNC119-binding protein C5orf30
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-UNC119-binding protein C5orf30 antibody
DescriptionRabbit polyclonal to UNC119-binding protein C5orf30
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Guinea pig, Cow, Pig, Chimpanzee, Non human primates, Gorilla, Orangutan
- Human thyroid gland tissue
General notesThe antibody solution should be gently mixed before use.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab150906 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
FunctionProbably plays a role in trafficking of proteins via its interaction with UNC119 and UNC119B cargo adapters: may help the release of UNC119 and UNC119B cargo or the recycling of UNC119 and UNC119B. May play a role in ciliary membrane localization via its interaction with UNC119B and protein transport into photoreceptor cells.
Sequence similaritiesBelongs to the UNC119-binding protein family.
Cellular localizationCytoplasm. Cell projection > cilium. Localizes to the transition zone and proximal cilium in addition to being found throughout the cytoplasm.
- Information by UniProt
- C5orf30 antibody
- CE030_HUMAN antibody
- chromosome 5 open reading frame 30 antibody
Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleoli and mitochondria. Recommended concentration of ab150906 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Lanes 2-3 : Anti-UNC119-binding protein C5orf30 antibody (ab150906)
Lane 1 : Marker
Lane 2 : HEK293T cell lysate - vector only transfected
Lane 3 : HEK293T cell lysate - overexpressing UNC119-binding protein C5orf30 with a C-terminal myc-DDK tag (~3.1kDa)
Immunohistochemical analysis of paraffin embedded Human thyroid gland tissue labeling UNC119-binding protein C5orf30 with ab150906 antibody at 1/200.
ab150906 has not yet been referenced specifically in any publications.