Key features and details
- Rabbit polyclonal to Unc5a
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Unc5a antibody
DescriptionRabbit polyclonal to Unc5a
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- ICC/IF: U-2 OS cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab222205 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionReceptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand.
Sequence similaritiesBelongs to the unc-5 family.
Contains 1 death domain.
Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 TSP type-1 domain.
Contains 1 ZU5 domain.
DomainThe ZU5 domain mediates the interaction with MAGED1, which participates in the induction of apoptosis.
modificationsPhosphorylated on cytoplasmic tyrosine residues. Phosphorylated by PKC in vitro.
Proteolytically cleaved by caspases during apoptosis. The cleavage does not take place when the receptor is associated with netrin ligand. Its cleavage by caspases is required to induce apoptosis.
Cellular localizationCell membrane. The interaction with PRKCABP regulates its surface expression and leads to its removal from surface of neurons and growth cones.
- Information by UniProt
- FLJ16449 antibody
- KIAA1976 antibody
- Netrin receptor UNC 5A antibody
ab222205 has not yet been referenced specifically in any publications.