Key features and details
- Mouse monoclonal [Guido-1] to UNC5B
- Suitable for: ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG2b
Product nameAnti-UNC5B antibody [Guido-1]
See all UNC5B primary antibodies
DescriptionMouse monoclonal [Guido-1] to UNC5B
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment, corresponding to amino acids 28-363 of Human UNC5B
This product was changed from ascites to tissue culture supernatant on 22nd May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesPurified from TCS.
Our Abpromise guarantee covers the use of ab54430 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
FunctionReceptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion (By similarity). Functions as netrin receptor that negatively regulates vascular branching during angiogenesis. Mediates retraction of tip cell filopodia on endothelial growth cones in response to netrin (By similarity). It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand (PubMed:12598906). Mediates apoptosis by activating DAPK1. In the absence of NTN1, activates DAPK1 by reducing its autoinhibitory phosphorylation at Ser-308 thereby increasing its catalytic activity.
Tissue specificityHighly expressed in brain. Also expressed at lower level in developing lung, cartilage, kidney and hematopoietic and immune tissues.
Sequence similaritiesBelongs to the unc-5 family.
Contains 1 death domain.
Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 2 TSP type-1 domains.
Contains 1 ZU5 domain.
modificationsPhosphorylated on cytoplasmic tyrosine residues.
Proteolytically cleaved by caspases during apoptosis. The cleavage does not take place when the receptor is associated with netrin ligand. Its cleavage by caspases is required to induce apoptosis.
Palmitoylation is required for pro-apoptotic activity, but not for location at lipid rafts.
Cellular localizationCell membrane. Membrane raft. Associated with lipid rafts.
- Information by UniProt
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ICC/IF image of ab54430 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54430, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This image was generated using the ascites version of the product.
ab54430 has been referenced in 4 publications.
- Srikanth P et al. Convergence of independent DISC1 mutations on impaired neurite growth via decreased UNC5D expression. Transl Psychiatry 8:245 (2018). PubMed: 30410030
- Miloudi K et al. Truncated netrin-1 contributes to pathological vascular permeability in diabetic retinopathy. J Clin Invest 126:3006-22 (2016). PubMed: 27400127
- Ramkhelawon B et al. Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity. Nat Med 20:377-84 (2014). IF . PubMed: 24584118
- König K et al. The uncoordinated-5 homolog B receptor affects hepatic ischemia reperfusion injury. PLoS One 7:e41085 (2012). Flow Cyt ; Mouse . PubMed: 22848430