Overview

  • Product name
  • Description
    Rabbit polyclonal to UNG
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity

    Predicted to work with: Mouse, Rat, Chicken, Human, Zebrafish
  • Immunogen

    Synthetic peptide:

    CRHFSKTNELLQKSGKKP

    , corresponding to amino acids 281-298 of Human UNG.

  • Positive control
    • HeLa cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    This antibody is affinity purified.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab13668 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 36.5 kDa).

Target

  • Function
    Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.
  • Tissue specificity
    Isoform 1 is widely expressed with the highest expression in skeletal muscle, heart and testicles. Isoform 2 has the highest expression levels in tissues containing proliferating cells.
  • Involvement in disease
    Defects in UNG are a cause of immunodeficiency with hyper-IgM type 5 syndrome (HIGM5) [MIM:608106]. Hyper-IgM syndrome is a condition characterized by normal or increased serum IgM concentrations associated with low or absent serum IgG, IgA, and IgE concentrations. HIGM5 is associated with profound impairment in immunoglobulin (Ig) class-switch recombination (CSR) at a DNA precleavage step.
  • Sequence similarities
    Belongs to the uracil-DNA glycosylase family.
  • Post-translational
    modifications
    Isoform 1 is processed by cleavage of a transit peptide.
  • Cellular localization
    Mitochondrion and Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DGU antibody
    • DKFZp781L1143 antibody
    • HIGM 4 antibody
    • HIGM4 antibody
    • OTTHUMP00000240527 antibody
    • OTTHUMP00000240528 antibody
    • OTTHUMP00000240529 antibody
    • UDG antibody
    • UNG 1 antibody
    • UNG 15 antibody
    • ung antibody
    • UNG_HUMAN antibody
    • UNG1 antibody
    • UNG15 antibody
    • UNG2 antibody
    • Uracil DNA glycosylase 1 antibody
    • Uracil DNA glycosylase 2 antibody
    • Uracil DNA glycosylase antibody
    • Uracil-DNA glycosylase antibody
    see all

Images

  • Detection of UNG in HeLa cell lysate with anti-UNG ab13668 at 2µg/ml dilution.

    Detection of UNG in HeLa cell lysate with anti-UNG ab13668 at 2µg/ml dilution.

References

This product has been referenced in:
  • Pauklin S  et al. Alternative induction of meiotic recombination from single-base lesions of DNA deaminases. Genetics 182:41-54 (2009). ICC/IF ; Caenorhabditis elegans . Read more (PubMed: 19237686) »
  • Di Noia JM  et al. Dependence of antibody gene diversification on uracil excision. J Exp Med 204:3209-19 (2007). WB ; Mouse . Read more (PubMed: 18070939) »
See all 3 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (293T cells)
Loading amount
10 µg
Specification
293T cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Jun 16 2006

Answer

Further to my e-mail last Friday regarding your problems with ab13668 (Rabbit polyclonal to UNG), I requested further information from the originator regarding its specificity. They advised me of a publication that specifically features the use of this antibody - please see figure 1D in Kruman et al., 2004 JBC 279 issue 42, p. 43952-43960. We will add this product specific reference to our datasheet and I will send the paper to you via a separate e-mail. Having reviewed this published data, I do think that this antibody detects UNG and will thus leave it in our range. As promised we will refund you for the cost of the antibody. There may just have been a problem with the vial you received or something that needs adjusting with your protocol or material under-investigation. I would still be interested to know the size of the two non-specific bands you find.

Read More

Answer

I've reviewed the datasheet for this product and see that the predicted band size for UNG is 36.5kD yet the WB image is showing two major bands, one at around 33kD and another at 55kDa while no explanation is provided for why the observed bands are different to the predicted size. I see you ordered this product on via Abcam order number 91966 on 11 July and paid by credit card. I will instruct our US accounts department to credit your card for the full cost of the order (USD 327.00). In the meantime, I would be interested to know the size of the two non-specific bands you find -- does your blot look similar to the one on our datasheet? I have also contacted the originator of the product to try to get to the bottom of why the observed bands are different to the expected. Interesingly we have sold quite a few units of this antibody over the last 3 months alone and yours is the first complaint we have received. I agree that there is something odd going on here and after a bit more digging, if I'm still not satisfied I will remove this product from our catalogue - our reputation is far too valuable to risk selling products that don't work. I look forward to hearing back from you.

Read More

Answer

Thank you for your enquiry regarding ab5197 and ab13668. Having done a BLAST search (for gallus gallus) for the immunogen sequences used to generate those antibodies I found: 92% homology for AID : Query: 1 NVRWAKGRRETYLC 14 N+RWAKGRRETYLC Sbjct: 17 NLRWAKGRRETYLC 30 88% homology for UNG: Query: 1 CRHFSKTNELLQKSGKKP 18 CRHFSKTNELL+KSGK+P Sbjct: 276 CRHFSKTNELLKKSGKEP 293 We have not tested these antibodies in chicken but should you choose to test them on your chicken B cells please let us know by sending us a review and we will reward you with 50 points on the Abcam Loyalty Scheme which can be redeemed on a number of vouchers. I hope this information helps, please do not hesitate to contact us if you need any more advice or information,

Read More

Answer

Thank you for your enquiry. By BLASTing the immunogen sequence (just click on the NCBI link located on our online datasheet), you can see that the sequence is conserved in both UNG1 and UNG2 (the immunogen corresponds to amino acids 281-298 of human UNG1 and amino acids 290-307 of human UNG2). The antibody recognizes a sequence that is 100% common to UNG1 and UNG2 and therefore would recognize both isoforms. The source of this antibody has made a great point. "Regarding the customer's desire for an UNG2 specific antibody, I don't know if there is one on the market that would just be specific for one isoform or the other. However, if the customer does a nuclear extraction, he would then only isolate the nuclear isoform - and in that case, this antibody could be used by the customer to detect UNG2 and not UNG1." If you have any additional questions, please let us know.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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