Anti-UNG antibody (ab233206)
Key features and details
- Rabbit polyclonal to UNG
- Suitable for: IHC-P, WB
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-UNG antibody
See all UNG primary antibodies -
Description
Rabbit polyclonal to UNG -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human, Pig -
Immunogen
Recombinant full length protein (His-T7-tag) corresponding to Human UNG aa 1 to the C-terminus. (Expressed in E.coli).
Database link: P13051 -
Positive control
- WB: Recombinant human UNG protein; Pig heart lysate. IHC-P: Human pancreas, cerebrum, glioma, liver, kidney and stomach tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233206 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 35 kDa.
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Notes |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 35 kDa. |
Target
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Function
Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine. -
Tissue specificity
Isoform 1 is widely expressed with the highest expression in skeletal muscle, heart and testicles. Isoform 2 has the highest expression levels in tissues containing proliferating cells. -
Involvement in disease
Defects in UNG are a cause of immunodeficiency with hyper-IgM type 5 syndrome (HIGM5) [MIM:608106]. Hyper-IgM syndrome is a condition characterized by normal or increased serum IgM concentrations associated with low or absent serum IgG, IgA, and IgE concentrations. HIGM5 is associated with profound impairment in immunoglobulin (Ig) class-switch recombination (CSR) at a DNA precleavage step. -
Sequence similarities
Belongs to the uracil-DNA glycosylase family. -
Post-translational
modificationsIsoform 1 is processed by cleavage of a transit peptide. -
Cellular localization
Mitochondrion and Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 7374 Human
- Omim: 191525 Human
- SwissProt: P13051 Human
- Unigene: 191334 Human
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Alternative names
- DGU antibody
- DKFZp781L1143 antibody
- HIGM 4 antibody
see all
Images
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Anti-UNG antibody (ab233206) at 2 µg/ml + Recombinant human UNG protein
Secondary
HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 35 kDa -
Anti-UNG antibody (ab233206) at 2 µg/ml + Pig heart lysate
Secondary
HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 35 kDa -
Formalin-fixed, paraffin-embedded human pancreas tissue stained for UNG using ab233206 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human cerebrum tissue stained for UNG using ab233206 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human glioma tissue stained for UNG using ab233206 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human liver tissue stained for UNG using ab233206 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human kidney tissue stained for UNG using ab233206 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human stomach tissue stained for UNG using ab233206 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab233206 has not yet been referenced specifically in any publications.