Anti-UNG antibody (ab245630)
Key features and details
- Rabbit polyclonal to UNG
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-UNG antibody
See all UNG primary antibodies -
Description
Rabbit polyclonal to UNG -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human UNG aa 263-313. The exact sequence is proprietary. NP_550433.1
Database link: P13051 -
Positive control
- WB: HeLa and HEK-293T whole cell lysates; IP: HeLa cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab245630 was affinity purified using an epitope specific to UNG immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab245630 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 35 kDa.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 35 kDa. |
Target
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Function
Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine. -
Tissue specificity
Isoform 1 is widely expressed with the highest expression in skeletal muscle, heart and testicles. Isoform 2 has the highest expression levels in tissues containing proliferating cells. -
Involvement in disease
Defects in UNG are a cause of immunodeficiency with hyper-IgM type 5 syndrome (HIGM5) [MIM:608106]. Hyper-IgM syndrome is a condition characterized by normal or increased serum IgM concentrations associated with low or absent serum IgG, IgA, and IgE concentrations. HIGM5 is associated with profound impairment in immunoglobulin (Ig) class-switch recombination (CSR) at a DNA precleavage step. -
Sequence similarities
Belongs to the uracil-DNA glycosylase family. -
Post-translational
modificationsIsoform 1 is processed by cleavage of a transit peptide. -
Cellular localization
Mitochondrion and Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 7374 Human
- Omim: 191525 Human
- SwissProt: P13051 Human
- Unigene: 191334 Human
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Alternative names
- DGU antibody
- DKFZp781L1143 antibody
- HIGM 4 antibody
see all
Images
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All lanes : Anti-UNG antibody (ab245630) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 35 kDa
Exposure time: 3 minutes
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab245630 has not yet been referenced specifically in any publications.