Overview

  • Product name

  • Description

    Rabbit polyclonal to UNG
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human UNG aa 263-313. The exact sequence is proprietary. NP_550433.1
    Database link: P13051

  • Positive control

    • WB: HeLa and HEK-293T whole cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab245630 was affinity purified using an epitope specific to UNG immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab245630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 35 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.
    • Tissue specificity

      Isoform 1 is widely expressed with the highest expression in skeletal muscle, heart and testicles. Isoform 2 has the highest expression levels in tissues containing proliferating cells.
    • Involvement in disease

      Defects in UNG are a cause of immunodeficiency with hyper-IgM type 5 syndrome (HIGM5) [MIM:608106]. Hyper-IgM syndrome is a condition characterized by normal or increased serum IgM concentrations associated with low or absent serum IgG, IgA, and IgE concentrations. HIGM5 is associated with profound impairment in immunoglobulin (Ig) class-switch recombination (CSR) at a DNA precleavage step.
    • Sequence similarities

      Belongs to the uracil-DNA glycosylase family.
    • Post-translational
      modifications

      Isoform 1 is processed by cleavage of a transit peptide.
    • Cellular localization

      Mitochondrion and Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • DGU antibody
      • DKFZp781L1143 antibody
      • HIGM 4 antibody
      • HIGM4 antibody
      • OTTHUMP00000240527 antibody
      • OTTHUMP00000240528 antibody
      • OTTHUMP00000240529 antibody
      • UDG antibody
      • UNG 1 antibody
      • UNG 15 antibody
      • ung antibody
      • UNG_HUMAN antibody
      • UNG1 antibody
      • UNG15 antibody
      • UNG2 antibody
      • Uracil DNA glycosylase 1 antibody
      • Uracil DNA glycosylase 2 antibody
      • Uracil DNA glycosylase antibody
      • Uracil-DNA glycosylase antibody
      see all

    Images

    • All lanes : Anti-UNG antibody (ab245630) at 0.1 µg/ml

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 35 kDa


      Exposure time: 3 minutes

    References

    ab245630 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab245630.
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