Product nameUniversal Lateral Flow Assay Kit
Universal Lateral Flow Assay Kit (ab270537) is designed to enable the easy development of customized sandwich lateral flow assays, by combining Ulfa-Tag kit and GOLD conjugation technologies with an immunochromatography test performed on Universal-LFA strips. The signal intensities can be qualitatively analyzed using the supplied scoring card or, for a quantitative detection, a LFA reader can be used.
The great advantage of this kit is its adaptability to any pair of capture and detection antibodies, which permits to detect of any type of analyte, without the need to strip the capture antibody onto LFA strips using expensive equipment.
This product is manufactured by Expedeon, an Abcam company. It was previously called Universal LFA Kit. Expedeon product code 4300-0100 is the same as the 100 test size of this product.
Tested applicationsSuitable for: Sandwich ELISAmore details
Storage instructionsPlease refer to protocols.
Components 100 tests 40nm GOLD-Biotin, 10 OD 1 vial 96-well clear low binding plates 2 units Gold 3 vials Gold Antibody Diluent 1 vial Gold Quencher Reagent 1 vial Gold Reaction Buffer 1 vial LL-Modifier 1 vial LL-Quencher 1 vial Scoring card 1 unit Ulfa-Tag mix 3 x 100µg Universal LFA strips 100 tests Universal Running Buffer 2 vials
Our Abpromise guarantee covers the use of ab270537 in the following tested applications.
|Sandwich ELISA||Use at an assay dependent concentration.|
The capture antibody is conjugated to Ulfa-Tag, and the detection antibody is conjugated to 40nm GOLD, both of which require only 30 seconds to set up.
The capture and detection antibodies are diluted and incubated with the analyte and then run on Universal LFA strips. Universal LFA strips consist of a nitrocellulose membrane containing a ‘Test line’ (T-line) of immobilized anti-Ulfa-Tag antibody, that binds the Ulfa-Tag conjugated capture antibody which further binds the analyte in complex with the GOLD-detection antibody. A red T-line appears when the analyte is present and the line intensity varies depending on the analyte concentration. Universal LFA strips also contain a ‘Control-line’ (C-line) that exploit the extraordinarily high affinity of streptavidin for biotin, which shows that the test is valid, and an absorbent pad to promote and control the flow of sample through the membrane.
Example of sandwich LFA assay for the detection of CRP in serum using the Universal Lateral Flow Assay kit: Ulfa-Tag and 40nm GOLD are used to conjugate the capture and detection antibody respectively. Results were analyzed using a Qiagen ESEQuant reader.
ab270537 has not yet been referenced specifically in any publications.