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Phone call with enquiries regarding the Universal Methyltransferase Activity Assay Kit (ab139433)
Asked on Mar 12 2013
As we discussed over the phone, I have looked into whether the Universal Methyltransferase Activity Assay Kit (ab139433) is likely to be suitable for the experiments you have been planning. The kit was developed to allow for the methyltransferase activity to be measured for biochemical assays using purified methyltransferase. The end user has to find their own enzyme/substrate to use in the assay.
It is theoretically possible to use the kit with biological samples however there are likely to be a number of problems encountered in this. You would not be able to target one enzyme as most substrates can be used with a variety of different methyltransferases (i.e. it would react with multiple enzymes in the sample). You would also need to optimize the cell culture samples as if there were the enzyme source and determine the optimal enzymatic kinetics required to get optimal window of activity when used with the substrate of your choice. However, any reductants will be an issue including DTT or any thiol containing sample/reagent as they will interfere with the assay.
Therefore we would not advise using this assay for biological samples. Unfortunately we do not currently have any alternative assay which would work with biological samples, appart form if you are particularly interested in the methyltransferase activity on Histon H3 K4, K9 or K27 (kits ab113452, ab113453 and ab113454).
Answered on Mar 12 2013