Overview

  • Product name

    Universal SIRT Activity Assay Kit (Colorimetric)
    See all SIRT1 kits
  • Detection method

    Colorimetric
  • Sample type

    Nuclear Extracts, Purified protein
  • Assay type

    Sandwich
  • Sensitivity

    > 1 ng
  • Range

    500 ng - 20000 ng
  • Assay time

    3h 30m
  • Species reactivity

    Reacts with: Human, Plants, Mammals, Fungi, Other species
  • Product overview

    The Universal SIRT Activity Assay Kit (Colorimetric) is a complete set of optimized buffers and reagents for measuring the activity/inhibition of total SIRT enzymes using nuclear extracts or purified SIRT isoforms (SIRTs 1-7) from a broad range of species such as mammals, plants, fungi, and bacteria, in a variety of forms including, but not limited to cultured cells and fresh and frozen tissues. Detection of inhibition or activation of SIRTs is important in elucidating mechanisms of epigenetic regulation of gene activation and silencing and may benefit diagnostics and therapeutics of cancer or neurological diseases. The Universal SIRT Activity Assay Kit (Colorimetric) provides the components to successfully achieve this.

  • Platform

    Microplate reader

Properties

Associated products

Images

  • Illustrated standard curve generated with SIRT assay standard.
  • Demonstration of high sensitivity of a SIRT activity assay achieved by using recombinant SIRT1 with ab156915.

Protocols

References

This product has been referenced in:

  • Chatterjee S  et al. CD38-NAD+Axis Regulates Immunotherapeutic Anti-Tumor T Cell Response. Cell Metab 27:85-100.e8 (2018). Read more (PubMed: 29129787) »
  • Guan Y  et al. Nicotinamide Mononucleotide, an NAD+ Precursor, Rescues Age-Associated Susceptibility to AKI in a Sirtuin 1-Dependent Manner. J Am Soc Nephrol 28:2337-2352 (2017). Read more (PubMed: 28246130) »
See all 5 Publications for this product

Customer reviews and Q&As

Answer

The inhibitor TSA included in the kit is used for inhibition of HDAC activity in order to allow that the measured activity is only from SIRTs.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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