Key features and details
- Rabbit polyclonal to UNK unkempt
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-UNK unkempt antibody
See all UNK unkempt primary antibodies
DescriptionRabbit polyclonal to UNK unkempt
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Pig, Chimpanzee, Gorilla
- HeLa, 293T, Jurkat and TCMK cell lysates.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab205021 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 88 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
Sequence similaritiesBelongs to the unkempt family.
Contains 5 C3H1-type zinc fingers.
Contains 1 RING-type zinc finger.
- Information by UniProt
- KIAA1753 antibody
- RING finger protein unkempt homolog antibody
- Unk antibody
Immunoprecipitation of UNK unkempt from 1 mg 293T cell lysates using 6 µg per reaction of positive control antibody (lane 1), ab205021 (lane 2) or control IgG (lane 3). For western blotting ab205021 was used at 0.4 µg/mL.
All lanes : Anti-UNK unkempt antibody (ab205021) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK whole cell lysate
Lane 5 : NIH-3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 88 kDa
Exposure time: 30 seconds
ab205021 has been referenced in 1 publication.
- Li N et al. Headcase and Unkempt Regulate Tissue Growth and Cell Cycle Progression in Response to Nutrient Restriction. Cell Rep 26:733-747.e3 (2019). PubMed: 30650363