Key features and details
- Rabbit polyclonal to Unrip
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Unrip antibody
See all Unrip primary antibodies
DescriptionRabbit polyclonal to Unrip
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Chimpanzee
- HeLa, 293T, Jurkat, TCMK1 and NIH 3T3 whole cell lysates.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab205015 was affinity purified using an epitope specific to Unrip immobilized on solid support.
Our Abpromise guarantee covers the use of ab205015 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 38 kDa.|
|IP||1/2 - 1/10.|
FunctionThe SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. STRAP may play a role in the cellular distribution of the SMN complex. Negatively regulates TGF-beta signaling but positively regulates the PDPK1 kinase activity by enhancing its autophosphorylation and by significantly reducing the association of PDPK1 with 14-3-3 protein.
Sequence similaritiesBelongs to the WD repeat STRAP family.
Contains 7 WD repeats.
Cellular localizationCytoplasm. Nucleus. Localized predominantly in the cytoplasm but also found in the nucleus.
- Information by UniProt
- MAP activator with WD repeats antibody
- MAWD antibody
- PTWD antibody
Detection of Unrip in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab205015 at 6 µg/mg lysate for IP (Lane 1). For WB detection ab205015 was used at 0.4 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds. Lysate prepared using NETN lysis buffer.
All lanes : Anti-Unrip antibody (ab205015) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK1 whole cell lysate
Lane 5 : NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 38 kDa
Exposure time: 10 seconds
Lysates prepared using NETN lysis buffer
ab205015 has not yet been referenced specifically in any publications.