Overview

  • Product name

  • Description

    Rabbit polyclonal to Unrip
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide within Human Unrip aa 300-350 (C terminal). The exact sequence is proprietary. NP_009109.3.
    Sequence:

    YGLWKCVLPEE DSGELAKPKI GFPETTEEEL EEIASENSDC IFPSAPDVKA


    Database link: Q9Y3F4

  • Positive control

    • HeLa, 293T, Jurkat, TCMK1 and NIH 3T3 whole cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7-8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab205015 was affinity purified using an epitope specific to Unrip immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab205015 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 38 kDa.
IP 1/2 - 1/10.

Target

  • Function

    The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. STRAP may play a role in the cellular distribution of the SMN complex. Negatively regulates TGF-beta signaling but positively regulates the PDPK1 kinase activity by enhancing its autophosphorylation and by significantly reducing the association of PDPK1 with 14-3-3 protein.
  • Sequence similarities

    Belongs to the WD repeat STRAP family.
    Contains 7 WD repeats.
  • Cellular localization

    Cytoplasm. Nucleus. Localized predominantly in the cytoplasm but also found in the nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • MAP activator with WD repeats antibody
    • MAWD antibody
    • PTWD antibody
    • Serine-threonine kinase receptor-associated protein antibody
    • serine/threonine kinase receptor associated protein antibody
    • strap antibody
    • STRAP_HUMAN antibody
    • UNR-interacting protein antibody
    • UNRIP antibody
    • WD 40 repeat protein PT WD antibody
    • WD-40 repeat protein PT-WD antibody
    see all

Images

  • Detection of Unrip in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab205015 at 6 µg/mg lysate for IP (Lane 1). For WB detection ab205015 was used at 0.4 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.  Lysate prepared using NETN lysis buffer.

  • All lanes : Anti-Unrip antibody (ab205015) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : TCMK1 whole cell lysate
    Lane 5 : NIH 3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 38 kDa


    Exposure time: 10 seconds


    Lysates prepared using NETN lysis buffer

References

ab205015 has not yet been referenced specifically in any publications.

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