Product nameUnstained Protein Ladder (10 - 200 kDa)
Tested applicationsSuitable for: WB, SDS-PAGEmore details
Unstained Protein Ladder ab234618 contains 12 unstained recombinant proteins covering a wide range of molecular weights from 10-200 kDa.
This unstained protein ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
Key features include:
- Broad range: 10-200 kDa
- Ready-to-use: Supplied in a loading buffer for direct loading on gels.
- Easily identified: The proteins resolve into clearly identifiable bands when separated on the SDS-PAGE gel (MES buffer), with the intensified 25 kDa and 85 kDa protein bands serving as the two reference bands.
5 µl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel and for Western transferring.
Learn more about our unstained and prestained protein ladders in our guide.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
Storage bufferpH: 7.5
Constituents: 0.44% Tris phosphate, 2% SDS, 0.003% DTT, 21.6% Urea, 15% Glycerol
Concentration information loading...
Our Abpromise guarantee covers the use of ab234618 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.
For monitoring protein transfer onto membranes and sizing of proteins on WB. Recommended loading amounts: 5 ul.
|SDS-PAGE||Use at an assay dependent concentration.
For monitoring protein migration and sizing of proteins on SDS-PAGE. Recommended loading amounts: 5 ul per loading for 15-well or 10-well mini-gel.
ab234618 has not yet been referenced specifically in any publications.