Unstained Protein Ladder (10 - 200 kDa) (ab234618)

Overview

  • Product name

    Unstained Protein Ladder (10 - 200 kDa)
  • Tested applications

    Suitable for: WB, SDS-PAGEmore details
  • General notes

    Unstained Protein Ladder ab234618 contains 12 unstained recombinant proteins covering a wide range of molecular weights from 10-200 kDa.

    This unstained protein ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

    Key features include:

    • Broad range: 10-200 kDa
    • Ready-to-use: Supplied in a loading buffer for direct loading on gels.
    • Easily identified: The proteins resolve into clearly identifiable bands when separated on the SDS-PAGE gel (MES buffer), with the intensified 25 kDa and 85 kDa protein bands serving as the two reference bands.

     5 µl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel and for Western transferring.

    Learn more about our unstained and prestained protein ladders in our guide.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
  • Storage buffer

    pH: 7.5
    Constituents: 0.44% Tris phosphate, 2% SDS, 0.003% DTT, 21.6% Urea, 15% Glycerol
  • Concentration information loading...

Applications

Our Abpromise guarantee covers the use of ab234618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.

For monitoring protein transfer onto membranes and sizing of proteins on WB. Recommended loading amounts: 5 ul.

SDS-PAGE Use at an assay dependent concentration.

For monitoring protein migration and sizing of proteins on SDS-PAGE. Recommended loading amounts: 5 ul per loading for 15-well or 10-well mini-gel.

Images

References

ab234618 has not yet been referenced specifically in any publications.

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