Recombinant Anti-uPA antibody [EP6274(2)] (ab169754)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP6274(2)] to uPA
- Suitable for: ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-uPA antibody [EP6274(2)]
See all uPA primary antibodies -
Description
Rabbit monoclonal [EP6274(2)] to uPA -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee -
Immunogen
Synthetic peptide within Human uPA aa 400 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P00749 -
Positive control
- ICC/IF: PC-3 cells; WB: PC-3 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP6274(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab169754 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/50 - 1/100.
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WB |
1/1000 - 1/5000. Predicted molecular weight: 48 kDa.
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Notes |
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ICC/IF
1/50 - 1/100. |
WB
1/1000 - 1/5000. Predicted molecular weight: 48 kDa. |
Target
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Relevance
Urokinase type plasminogen activator (uPA) is secreted from cells as a precursor which is activated to the two chain form consisting of an A and B chain. The active high MW form is further processed by removal of an amino terminal fragment to an active low MW form (35 kDa). uPA is a serine protease that activates plasminogen toplasmin. High levels of uPA and plasminogen activator inhibitor type 1 (PAI 1) in breast cancer tissue extracts have been associated with rapid disease progression. The malignant phenotype of prostatic tumor cells correlates with the expression of both uPA and its cell membrane receptor (uPAR). -
Database links
- Entrez Gene: 738078 Chimpanzee
- Entrez Gene: 5328 Human
- Omim: 191840 Human
- SwissProt: P00749 Human
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Alternative names
- ATF antibody
- BDPLT5 antibody
- EC 3.4.21.73 antibody
see all
Images
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Anti-uPA antibody [EP6274(2)] (ab169754) at 1/1000 dilution (Purified) + PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
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Immunocytochemistry analysis of PC-3 (Human prostate adenocarcinoma epithelial cell) cells labeling uPA with Purified ab169754 at 1:50 dilution (3.78 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488,ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab169754 has been referenced in 14 publications.
- Lottin M et al. Molecular Landscape of the Coagulome of Oral Squamous Cell Carcinoma. Cancers (Basel) 14:N/A (2022). PubMed: 35053621
- Ran Q et al. Hypermethylation of the Promoter Region of miR-23 Enhances the Metastasis and Proliferation of Multiple Myeloma Cells via the Aberrant Expression of uPA. Front Oncol 12:835299 (2022). PubMed: 35707350
- Fang L et al. PLAU directs conversion of fibroblasts to inflammatory cancer-associated fibroblasts, promoting esophageal squamous cell carcinoma progression via uPAR/Akt/NF-?B/IL8 pathway. Cell Death Discov 7:32 (2021). PubMed: 33574243
- Sun J et al. Long non-coding RNA TRPM2-AS regulates microRNA miR-138-5p and PLAU (Plasminogen Activator, Urokinase) to promote the progression of gastric adenocarcinoma. Bioengineered 12:9753-9765 (2021). PubMed: 34696681
- Gaweda-Walerych K et al. Two Rare Variants in PLAU and BACE1 Genes-Do They Contribute to Semantic Dementia Clinical Phenotype? Genes (Basel) 12:N/A (2021). PubMed: 34828412