Overview

  • Product name

    Anti-uPA antibody [U-16]
    See all uPA primary antibodies
  • Description

    Mouse monoclonal [U-16] to uPA
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ELISA, IHC-Fr, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Native human uPA (NP_002649.1).

  • Epitope

    ab131433 binds to the B-chain of uPA

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituents: 99% PBS, 0.82% Sodium chloride
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    U-16
  • Myeloma

    NS1/1-Ag4-1
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab131433 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Use under non reducing condition. Predicted molecular weight: 48 kDa.
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use 0.1-1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance

    Urokinase type plasminogen activator (uPA) is secreted from cells as a precursor which is activated to the two chain form consisting of an A and B chain. The active high MW form is further processed by removal of an amino terminal fragment to an active low MW form (35 kDa). uPA is a serine protease that activates plasminogen toplasmin. High levels of uPA and plasminogen activator inhibitor type 1 (PAI 1) in breast cancer tissue extracts have been associated with rapid disease progression. The malignant phenotype of prostatic tumor cells correlates with the expression of both uPA and its cell membrane receptor (uPAR).
  • Database links

  • Alternative names

    • ATF antibody
    • BDPLT5 antibody
    • EC 3.4.21.73 antibody
    • PLAU antibody
    • QPD antibody
    • u PA antibody
    • U plasminogen activator antibody
    • u-PA antibody
    • UPA antibody
    • UPAM antibody
    • Urinary plasminogen activator antibody
    • URK antibody
    • Urokinase plasminogen activator antibody
    • Urokinase type plasminogen activator antibody
    see all

Images

  • Overlay histogram showing HT1080 cells stained with ab131433 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab131433, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HT1080 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References

This product has been referenced in:

  • Li H & Chen C Quercetin Has Antimetastatic Effects on Gastric Cancer Cells via the Interruption of uPA/uPAR Function by Modulating NF-?b, PKC-d, ERK1/2, and AMPKa. Integr Cancer Ther 17:511-523 (2018). Read more (PubMed: 28627240) »
  • Albaret MA  et al. Externalized Keratin 8: A Target at the Interface of Microenvironment and Intracellular Signaling in Colorectal Cancer Cells. Cancers (Basel) 10:N/A (2018). Read more (PubMed: 30453567) »
See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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