Product nameAnti-uPA Receptor/U-PAR antibody
See all uPA Receptor/U-PAR primary antibodies
DescriptionRabbit polyclonal to uPA Receptor/U-PAR
Tested applicationsSuitable for: WB, IHC-P, ICCmore details
Species reactivityReacts with: Mouse, Rat, Human
- Human placenta, Rat brain, Rat thymus and Rat heart tissue lysates; MCF7, SMMC, HeLa, Raji and Colo320 whole cell lysates; MCF7 cells
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab103791 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 37 kDa.|
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC||Use a concentration of 1 µg/ml.|
FunctionActs as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form.
Tissue specificityExpressed in neurons of the rolandic area of the brain (at protein level). Expressed in the brain.
Sequence similaritiesContains 3 UPAR/Ly6 domains.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- CD 87 antibody
- CD87 antibody
- CD87 antigen antibody
All lanes : Anti-uPA Receptor/U-PAR antibody (ab103791) at 1 µg/ml
Lane 1 : Human placenta tissue lysate
Lane 2 : Rat brain tissue lysate
Lane 3 : Rat thymus tissue lysate
Lane 4 : Rat heart tissue lysate
Lane 5 : MCF7 whole cell lysate
Lane 6 : SMMC whole cell lysate
Lane 7 : HeLa whole cell lysate
Lane 8 : Raji whole cell lysate
Lane 9 : Colo320 whole cell lysate
Predicted band size: 37 kDa
ab103791, at 1µg/ml, staining uPA Receptor/U-PAR in MCF7 cells by Immunocytochemistry.
MDA-MB-231 cells were incubated at 37°C for 24h with vehicle control (0 µM) and varied concentrations of SB 203580 hydrochloride (ab120235). Decreased expression of uPA receptor/U-PAR in MDA-MB-231 cells correlates with an increase in SB 203580 hydrochloride concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab103791 at 1 µg/ml and ab1801 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.
This product has been referenced in:
- Wach S et al. Exploring the MIR143-UPAR Axis for the Inhibition of Human Prostate Cancer Cells In Vitro and In Vivo. Mol Ther Nucleic Acids 16:272-283 (2019). Read more (PubMed: 30933831) »
- Huang JM et al. Ulinastatin Inhibits Osteoclastogenesis and Suppresses Ovariectomy-Induced Bone Loss by Downregulating uPAR. Front Pharmacol 9:1016 (2018). Read more (PubMed: 30245631) »