Overview

  • Product name

    Anti-uPA Receptor/U-PAR antibody
    See all uPA Receptor/U-PAR primary antibodies
  • Description

    Goat polyclonal to uPA Receptor/U-PAR
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IP, Neutralisingmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human uPA Receptor/U-PAR.

Properties

Applications

Our Abpromise guarantee covers the use of ab3129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 55 kDa.
IP Use a concentration of 5 - 10 µg/ml.
Neutralising Use a concentration of 2 - 4 µg/ml.

Target

  • Function

    Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form.
  • Tissue specificity

    Expressed in neurons of the rolandic area of the brain (at protein level). Expressed in the brain.
  • Sequence similarities

    Contains 3 UPAR/Ly6 domains.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 87 antibody
    • CD87 antibody
    • CD87 antigen antibody
    • MO 3 antibody
    • MO3 antibody
    • Monocyte activation antigen Mo3 antibody
    • Plasminogen activator receptor urokinase antibody
    • Plasminogen activator urokinase receptor antibody
    • PLAUR antibody
    • U PAR antibody
    • u plasminogen activator receptor antibody
    • U-PAR antibody
    • u-plasminogen activator receptor form 2 antibody
    • UPA receptor antibody
    • uPAR antibody
    • UPAR_HUMAN antibody
    • Urinary plasminogen activator receptor antibody
    • URKR antibody
    • Urokinase plasminogen activator receptor antibody
    • Urokinase plasminogen activator surface receptor antibody
    • urokinase-type plasminogen activator (uPA) receptor antibody
    see all

References

This product has been referenced in:

  • Pavet V  et al. Plasminogen activator urokinase expression reveals TRAIL responsiveness and supports fractional survival of cancer cells. Cell Death Dis 5:e1043 (2014). WB . Read more (PubMed: 24481457) »
  • Al-Souhibani N  et al. The RNA-binding zinc-finger protein tristetraprolin regulates AU-rich mRNAs involved in breast cancer-related processes. Oncogene : (2010). WB ; Human . Read more (PubMed: 20498646) »
See all 3 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for your email. The lot number customer used in 2007 was different than you have been using. So we would expect some variations due to cell line or other lab related factors. I can assure you that there is no change in the antibody itself other than difference in lot. We are also not sure whether this antibody has any lot related issues, so I am sorry I am unable to comment on this. I would also like to ask is this antibody able to neutralize the effect of uPA? I hope this information will help. Should you have any other question please do not hesitate to ask.

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Answer

Thank you for contacting us. Could you specify are the cells just dying or there is some kind of fungus or bacterial growth? The antibody solution only contain sodium azide as cytotoxic compound however the affect of this would be minimal at the Abcam recommended dilutions. The recommended concentration and dilutions to use is between 2-4 ug/ml. However this is for guideline purpose only. The dilutions have to be determined by end user because the conditions vary from cells to cells. We would recommend using a range of dilution e.g. 0.5 ug/ml, 1 ug/ml and 2 ug/ml in order to get optimized neutralization in the cell line you have been using. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your enquiry. Ab3129 has not yet been tested for cross-reactivity with mouse uPA, so I would suggest a control from human samples. For example, uPA and uPAR are known to be overexpressed in mesenchymal and epithelial tumor cells. If you have any additional questions, please contact us again.

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